| 摘要: |
| 【目的】研究秦川牛、南阳牛、鲁西牛和安格斯牛共630头个体的单磷酸腺苷激活的蛋白激酶α2亚基(AMPKα2)基因PRKAA2的多态性，并分析其多态性与200头秦川牛生长及肉质性状的关联性。【方法】利用PCR-SSCP、DNA测序、飞行质谱(MALDI-TOF)等技术，分析检测4个品种牛PRKAA2基因6个外显子的单核酸多态性（SNPs）；采用生物信息学方法结合统计分析软件，分析秦川牛各多态位点的遗传变异特性与体尺及肉质性状的关联性。【结果】PRKAA2基因第2、4、6、7、8、9外显子中，只检测到第4外显子27 G>C和60 T>C 2个突变位点；关联分析表明，27 G>C位点与秦川牛背膘厚和眼肌面积显著关联(P<0.05)；60 T>C位点与秦川牛体斜长极显著相关(P<0.01），与体高、尻长、腰角宽、坐骨端宽、眼肌面积显著相关(P<0.05)。【结论】PRKAA2基因对秦川牛部分体尺及肉质性状有极显著或显著影响，可作为秦川肉牛新品种早期选择的候选基因和分子辅助标记。 |
| 关键词: 秦川牛 PRKAA2基因 单核酸多态性（SNPs） 体尺性状 肉质性状 |
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| 基金项目:国家863计划项目（2013AA102505）；国家自然科学基金项目（31272411）；陕西省自然科学基金项目（2007C127）；陕西省科学技术发展计划项目（2011K01-09） |
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| SNPs of bovine PRKAA2 gene and its association with growth and meat quality traits |
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TIAN Wanqiang,MEI Chugang,FU Changzhen,et al
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| Abstract: |
| 【Objective】Polymorphism of PRKAA2 gene on 630 individuals from four cattle breeds (Qinchuan,Nanyang,Luxi and Angus) was detected to analyze its genetic structures and varieties.The association of different genotypes with growth and meat quality traits of 200 Qinchuan cattle was also analyzed.【Method】SNPs located in exon 2,exon 4,exon6,exon 7,exon 8 and exon 9 were detected in PRKAA2 gene of four cattle breeds by PCR-SSCP,random DNA sequencing method and MALDI-TOF technology.Genetic variation of SNPs and the association of different genotypes with growth and meat quality traits of Qinchuan cattle were also analyzed by bioinformatics method and SPSS software.【Result】Two SNPs (27 G>C and 60 T>C) located in only exon 4 were detected in 6 exons of PRKAA2 gene.The 27 G>C SNP was associated with back fat thickness (BF) and loin muscle area (LMA) (P<0.05) significantly.The 60 T>C SNP was associated with body length (BL) (P<0.01) extremely significantly,and with withers height (WH),rump length (RL),hip width (HW),pin bone width (PBW) and LMA (P<0.05) significantly.【Conclusion】PRKAA2 gene had potential effects on growth and meat quality traits of Qinchuan cattle and could be used for candidate gene and marker-assisted selection (MAS) in early selection of new varieties of Qinchuan cattle. |
| Key words: Qinchuan cattle PRKAA2 gene,SNPs growth traits meat quality traits |
