| 摘要: |
| 【目的】揭示云斑天牛对寄主核桃的选择机制，为筛选对云斑天牛具有生物活性的植物源引诱剂，实现对该虫的生态控制提供理论指导。【方法】采用顶空固相微萃取（Headspace solid phase micro-extraction，HS-SPME）结合气相色谱 质谱（Gas chromatograph-mass spectrometry，GC-MS）联用技术，分析了核桃树皮和树叶中的挥发性成分。【结果】核桃树皮和树叶共含有萜烯类、烃类、芳香族化合物、醇类、醛类、酮类、酯类、醌类和杂环化合物9类65种挥发性成分。其中，核桃树皮挥发性成分主要包括9类36种，分别是15种萜烯类、4种烃类、4种芳香族化合物、1种醇类、3种醛类、1种酮类、2种酯类、3种醌类和3种杂环化合物。核桃树叶挥发性成分主要包括8类42种，分别是22种萜烯类、2种烃类、4种芳香族化合物、4种醇类、2种醛类、1种酮类、5种酯类和2种醌类。核桃树皮和树叶均含有的挥发性成分共4类13种，分别是9种萜烯类、1种醛类、1种酯类和2种醌类。此外，核桃树皮和树叶萜烯类相对含量最高，分别为43.371%和78.948%；核桃树皮烃类相对含量最低，为1.043%；核桃树叶酮类和烃类相对含量均较低，分别为0.009%和0.268%。【结论】云斑天牛寄主核桃树皮和树叶挥发性成分主要是萜烯类、烃类、芳香族化合物、醇类、醛类、酮类、酯类、醌类和杂环化合物，其中萜烯类相对含量最高，酮类和烃类相对含量均较低。 |
| 关键词: 核桃树皮 核桃树叶 顶空固相微萃取 气相色谱 质谱联用 挥发性成分 |
| DOI： |
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| 基金项目:国家自然科学基金项目（31270694）；四川农业大学“211”工程双支计划资助项目（00370101） |
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| Volatile components in bark and leaf of host plant Juglans regia L. of Batocera lineolata Chevrolat |
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ZHUO Zhihang,YANG Wei,XU Danping,et al
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| Abstract: |
| 【Objective】The volatile components in bark and leaf of host plant Juglans regia L.of Batocera lineolata Chevrolat were determined to explore the selection mechanism of Batocera lineolata Chevrolat on host plants and provide references for screening effective attractants of plant origin and ecological control of Batocera lineolata Chevrolat.【Method】Headspace solid phase micro-extraction (HS-SPME) combined with gas chromatograph mass spectrometry (GC-MS) was selected to determine volatile components in bark and leaf of Juglans regia L.【Result】Bark and leaf of Juglans regia L.contained a total of 65 volatile components from 9 groups.The bark contained 36 volatile components from 9 groups including 15 terpenes,4 hydrocarbons,4 aromatic compounds,1 alcohol,3 aldehydes,1 ketone,2 esters,3 quinones and 3 heterocyclic compounds.The leaf contained 42 volatile components from 8 groups including 22 terpenes,2 hydrocarbons,4 aromatic compounds,4 alcohols,2 aldehydes,1 ketone,5 esters and 2 quinones.The bark and leaf shared 13 volatile components from 4 groups including 9 terpenes,1 aldehyde,1 ester and 2 quinones.The relative contents of terpene were the highest in both bark (43.371%) and leaf (78.948%).Hydrocarbons had the lowest content of 1.043% in bark,while both ketones (0.009%) and hydrocarbons (0.268%) were low in leaf.【Conclusion】The volatile components in bark and leaf of host plant Juglans regia L.of Batocera lineolata Chevrolat included terpenes,hydrocarbons,aromatic compounds,alcohols,aldehydes,ketones,esters,quinones and heterocyclic compounds,among which terpenes had highest content while the contents of ketones and hydrocarbons were lower. |
| Key words: Juglans regia L. bark Juglans regia L. leaf HS-SPME GC-MS volatile components |
