| 摘要: |
| 【目的】研究凤丹种子胚乳中内源抑制物质活性及其成分组成,为了解其休眠机理提供参考。【方法】采用系统溶剂分离法，分离萃取凤丹胚乳中的内源抑制物质，对4种有机溶剂（石油醚、乙酸乙酯、乙醚、甲醇）萃取液和水相进行白菜种子发芽试验，确定发挥抑制作用的优势组分，并对其成分进行GC-MS分析。【结果】凤丹种子胚乳各有机溶剂萃取液对白菜种子发芽率和幼苗苗高生长、根长生长均有不同程度的抑制作用，但抑制物质活性存在一定差异。4种有机溶剂萃取液中抑制作用最强的是乙醚组分，其次是甲醇组分，再次是乙酸乙酯组分。利用GC-MS方法从乙醚组分中共检出6种物质，主要为乙酸(66.661%)、甲酸(25.996%)、乙酐(2.399%)、苯酚(2.217%)等；从乙酸乙酯组分中共检出11种物质，主要为乙酸(56.343%)、甲酸(14.772%)、DL-3-乙基乙酸丁酸酯(6.266%)、乙酐(4.834%)等；从甲醇组分中共检出33种物质，主要为苯甲酸(32.631%)、1,3-二羟基丙酮(10.997%)、5-羟甲基糠醛(8.964%)、乙酸(5.751%)等；这些物质中被证实有抑制活性的是乙酸和苯甲酸；GC-MS检出物主要为有机酸类、酮类、酯类和醛类等，不同组分中相对含量高的均为有机酸类物质。【结论】乙醚萃取液中的化合物可能是导致凤丹种子萌发受抑制的主要物质。 |
| 关键词: 凤丹 胚乳 内源抑制物质 GC-MS |
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| 基金项目:吉林省科技发展计划项目（2013020607NY） |
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| Activities and GC MS identification of endogenous inhibitory substances in endosperm of Paeonia ostii |
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SUN Xiaogang,DING Yan,GUO Taijun,et al
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| Abstract: |
| 【Objective】This paper studied activities and components of inhibitory substances in seed endosperm of Paeonia ostii to understand the dormancy mechanism.【Method】System solvent separation was used to extract endogenous inhibiting substances in endosperm of P.ostii with four organic solvents (petroleum ether,ethyl acetate,ethyl ether,methanol) and water,and seed germination of Chinese cabbage was tested.Then,the dominant components of inhibitory substances were determined using GC-MS analysis.【Result】The seed germination rate and height and root length of seedling of Chinese cabbage were inhibited by organic solvent extracts of inhibitory substances in seed endosperm of P.ostii.There were differences in the inhibition effects with a decreasing order of ethyl ether>methanol>ethyl acetate>petroleum ether.Six substances including acetic acid (66.661%),formic acid (25.996%),acetic anhydride (2.399%),and phenol (2.217%) were detected by GC-MS from ether extract.Eleven were detected from ethyl acetate extract including acetic acid (56.343%),formic acid (14.772%),ethyl (+)-3-acetoxybutyrate (6.266%),and acetic anhydride (4.834%).Thirty-three substances including benzoic acid (32.631%),1,3-dihydroxy-2-propanone (10.997%),5-(hydroxymethyl)-2-Furancar-boxaldehyde (8.964%),and acetic acid (5.751%) were obtained from methanol extract.The substances with inhibitory effects were acetic acid and benzoic acid.【Conclusion】The compounds identified in the ether extract may be the main substances inhibiting seed germination of P.ostii. |
| Key words: Paeonia ostii endosperm inhibitory substance GC-MS |
