| 摘要: |
| 【目的】杜仲橡胶是一种多萜类物质，而甲羟戊酸（MVA）途径是合成萜类物质的重要途径之一。分析杜仲MVA途径相关基因表达的差异，预测杜仲橡胶合成的主要上游途径。【方法】在杜仲幼果和叶片转录组测序的基础上，根据基因功能注释和表达分析，确定MVA途径的系列基因，并对其进行表达差异分析。【结果】杜仲橡胶合成的MVA途径共涉及23条Unigene，分别为3条EuACOT、3条EuHMGS、8条EuHMGR、2条EuMK、1条EuPMK和6条EuMDP基因。根据转录组RPKM(Reads Per Kilo bases per Million reads)数据对基因表达差异进行分析，结果表明杜仲MVA途径中的EuACOT8、EuACOT10、EuHMGS4、EuHMGS5、EuHMGS6、EuHMGR18、EuMK3、EuMK4、EuMDP7、EuMDP8、EuMDP9、EuMDP10、EuMDP11基因在幼果和叶片中的表达量具有显著差异，其中仅EuMDP9在叶片中的表达量大于幼果，而其余基因在幼果的表达量大于叶片；EuHMGR15和EuHMGR17只在幼果中特异表达。【结论】MVA途径绝大部分基因在果实中大量表达，这与果实中杜仲橡胶含量远超过叶片的现象相吻合，推测杜仲果实MVA途径为杜仲橡胶合成的主要途径。 |
| 关键词: 杜仲 幼果和叶片 甲羟酸途径 基因表达差异 |
| DOI： |
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| 基金项目:国家林业公益性行业科研专项（201004029） |
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| Differential expression of MVA pathway genes in young fruits and leaves of Eucommia ulmoides Oliver |
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WANG Lin,WUYUN Tana,YE Shengjing
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| Abstract: |
| 【Objective】Eucommia ulmoides Rubber (EuR) is one type of terpenoids,which can be synthesized by mevalonate (MVA) pathway.This study analyzed the differential expression of genes in MVA pathway in young fruits and leaves of E.ulmoides oliver and predicted main pathways of EuR synthesis.【Method】According to the high-throughput transcriptome sequencing (RAN-seq) data,functional annotation and expression analysis in E.ulmoides young fruits and leaves,unigenes of MVA pathway were determined,and their expressions were systematically analyzed.【Result】Twenty-three unigenes in MVA pathway were annotated including 3 acetyl-CoA C-acetyltransferase (EuACTO),3 hydroxymethylglutaryl-CoA synthase (EuHMGS),8-hydroxymethylglutaryl-CoA reductase (EuHMGR),2 mevalonate kinase (EuMK),1 phosphomevalonate kinase (EuPMK),and 6 diphosphomevalonate decarboxylase (EuMDP).Reads Per Kilo bases per Million reads (RPKM) data analysis showed that the expressions of EuACOT8,EuACOT10,EuHMGS4,EuHMGS5,EuHMGS6,EuHMGR18,EuMK3,EuMK4,EuMDP7,EuMDP8,EuMDP9,EuMDP10,and EuMDP11 were significantly different in young fruits and leaves.Only the expression of EuMDP9 in leaves was greater than in young fruits,while the expressions of other genes in young fruits were greater than in leaves.Furthermore,EuHMGR15 and EuHMGR17 were unique in young fruits.【Conclusion】Most of genes in MVA pathway were abundantly expressed in young fruits,which was consistent with the fact that rubbers form fruits were more than from leaves.Therefore,MVA pathway was considered the main synthetic pathway of EuR. |
| Key words: Eucommia ulmoides Olives young fruits and leaves MVA pathway differential expression of gene |
