| 摘要: |
| 【目的】对苹果疏除幼果多糖进行分离与纯化，并对所得多糖组分TYAP-2进行结构表征及抗氧化活性分析。【方法】采用热水浸提法从苹果疏除幼果中提取水溶性粗多糖TYAP，并经DEAE-52纤维素柱层析和Sephadex G-150凝胶柱层析分离纯化，获得一种新的多糖组分TYAP-2。通过高效液相色谱（HPLC）、紫外光谱（UV）、傅里叶变换红外光谱（FT-IR）和环境电镜扫描（ESEM）等方法研究了TYAP-2组成及分子结构，并对其体外抗氧化活性进行了初步探讨。【结果】HPLC分析表明，TYAP-2是分子质量约为479 ku的均一多糖，阿拉伯糖、半乳糖和葡萄糖是组成TYAP-2的主要单糖，而鼠李糖、半乳糖醛酸、葡萄糖醛酸和木糖的含量较少；FT-IR分析表明，TYAP-2中存在D-吡喃葡萄糖环结构；ESEM检测表明，TYAP-2呈长杆状和片层状2种结构；抗氧化分析表明，当质量浓度为5.0 mg/mL时，TYAP-2对DPPH·的清除能力为65.08%，总还原力（OD值）为0.603。【结论】TYAP-2是一种可以作为天然植物抗氧化剂的多糖组分。 |
| 关键词: 苹果疏除幼果 多糖TYAP-2 分离纯化 结构特性 抗氧化活性 |
| DOI： |
| 分类号: |
| 基金项目:农业部现代苹果产业技术体系专项基金项目（CARS-28） |
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| Purification and characteristics of polysaccharide TYAP-2 from thinned young apples |
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DOU Jiao,GUO Yu-rong,LI Jie,et al
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| Abstract: |
| 【Objective】This study purified TYAP-2 from thinned young apples and analyzed its characteristics and antioxidant activities.【Method】The crude polysaccharides TYAP was extracted from thinned-young apples by hot water method and further fractionated by DEAE-52 cellulose and sephadex G-150 to obtain a new polysaccharide component named TYAP-2.Then,the structural characteristics of TYAP-1 were analyzed by high performance liquid chromatography (HPLC),ultraviolet spectrum (UV),Fourier transform infrared spectrometer (FT-IR) and environment scanning electron microscopy (ESEM) methods.In addition,the antioxidant activities of TYAP-2 in vitro were evaluated.【Result】HPLC analysis indicated that TYAP-2 was a homogeneous polysaccharide with an average molecular weight of 479 ku.TYAP-2 mainly consisted of glucose,galactose and arabinose,while rhamnose,galacturonic acid,glucuronic acid and xylose presented in trace amounts.IR spectral analysis suggested the presence of pyranose ring in TYAP-2.Moreover,the flake and rods morphologies of TYAP-2 were detected by ESEM method.The scavenge ability of TYAP-2 with concentration of 5.0 mg/mL against DPPH· was 65.08% and the OD was 0.603.【Conclusion】TYAP-2 is a polysaccharide that can be used as a natural plant antioxidant. |
| Key words: thinned-young apple polysaccharides TYAP-2 purification structure characterization antioxidant activities |
