| 摘要: |
| 【目的】明确陕西关中地区小菜蛾田间种群对9种常用杀虫剂的抗药性现状，为抗性治理和田间有效防治小菜蛾提供依据。【方法】在室内采用生物测定法和区分剂量法，测定2012-2013年间陕西杨凌、宝鸡和渭南3个地区小菜蛾田间种群对阿维菌素、高效氯氰菊酯、丁醚脲、啶虫隆、溴虫腈、茚虫威、多杀菌素、Bt毒素Cry1Ac和氯虫苯甲酰胺9种常用杀虫剂的敏感性。【结果】陕西3个地区的小菜蛾田间种群对大部分供试药剂都产生了不同程度的抗性。宝鸡、渭南和杨凌种群均对高效氯氰菊酯产生了高-极高水平抗性，对阿维菌素产生了中等-高水平抗性，对溴虫腈和啶虫隆产生了低-中等水平抗性，对茚虫威、多杀菌素和氯虫苯甲酰胺的抗性水平相对较低，处于敏感-低水平抗性。对丁醚脲和Bt毒素的抗性各地差异较大，对丁醚脲的抗性，杨凌种群处于低-中等水平抗性，渭南种群处于中等水平抗性，宝鸡种群处于敏感性下降；对Bt毒素的抗性，宝鸡种群处于中等水平抗性，杨凌和渭南种群处于敏感性下降-低水平抗性。【结论】3个地区应停止使用已产生高水平抗性的高效氯氰菊酯和阿维菌素，减少已产生中等水平抗性药剂的使用次数，处于低水平抗性的杀虫剂可作为防治小菜蛾的主要药剂。在杀虫剂使用过程中应注意药剂的轮换使用，以延缓小菜蛾抗药性加剧。 |
| 关键词: 小菜蛾 杀虫剂 抗药性 |
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| 基金项目:农业部公益性行业科研专项（201103021）；国家科技支撑计划项目（2012BAD19B06） |
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| Resistance status of diamondback moth Plutella xylostella (L.) to nine insecticides in Shaanxi |
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YIN Shao-xin,ZHANG Chun-ni,ZHANG Ya-lin,et al
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| Abstract: |
| 【Objective】This study uncovered the resistance status of diamondback moth (DBM),Plutella xylostella (L.),to nine commonly used insecticides in Shaanxi to provide basis for effective management of DBM in field.【Method】The sensitivities of DBM field populations in Yangling,Baoji and Weinan in Shaanxi province to nine insecticides including abamectin,α-cypermethrin,diafenthiuron,chlorfluazuron,chlorfenapyr,indoxcarb,spinosad,chlorantraniliprole and insecticidal protein Cry1Ac from Bacillus thuringiensis (Bt) were tested in 2012 and 2013 by the dose-response line bioassay and the discriminating dose bioassay in laboratory.【Result】DBM field populations collected from the three different regions during 2012-2013 have developed different levels of resistance to most insecticides.Baoji,Weinan and Yangling populations have developed high to very high levels of resistance to α-cypermethrin, moderate to high levels of resistance to abamectin,low to moderate levels of resistance to chlorfenapyr and chlorfluazuron,and kept susceptible to low levels of resistance to indoxcarb,spinosad and chlorantraniliprole.There were large differences in resistance to Bt and diafenthiuron among the tested field populations.Decreased sensitivity,low to moderate and moderate resistance to diafenthiuron were observed in Baoji,Yangling and Weinan populations,respectively.Yangling and Weinan populations displayed decreased sensitivity or low levels of resistance to Bt,whereas Baoji populations exhibited moderate levels of resistance to Bt.【Conclusion】Applying α-cypermethrin and abamectin for control of DBM in these three regions should be discontinued because of high levels of resistance.The spraying times should be reduced for insecticides,to which DBM populations had developed moderate levels of resistance.The insecticides remained susceptible or with low levels of resistance can be used as the alternative insecticides to manage DBM populations.Furthermore,insecticides should be alternatively used to delay the development of resistance. |
| Key words: Plutella xylostella insecticide resistance |
