| 摘要: |
| 【目的】从油菜根际土壤中筛选油菜亲和性根际菌株，以期获得油菜专用优良特异性根际促生菌。【方法】采用常规稀释平板法从油菜根际筛选油菜根际细菌，提取油菜凝集素进行复筛，获得油菜亲和性根际细菌，测定复筛菌株的产铁载体、吲哚乙酸（IAA）能力，以及1-氨基环丙烷-1-羧酸（ACC）脱氨酶和溶磷活性，采用盆栽试验评价菌株对油菜的促生效果。【结果】经过复筛获得油菜亲和性根际细菌32株，其中30株菌株具有产铁载体能力，16株菌株的产铁载体能力达到5+水平；29株菌株具有溶磷活性，7株菌株产量达到100.00 μg/mL；16株菌株能产IAA，菌株P17的产量最高，达到30.48 mg/L；7株菌株具有ACC脱氨酶活性。盆栽试验表明，有25株菌株具有不同程度的促生作用，N10和K6促生作用显著，22株菌株的促生作用达到施全肥水平。【结论】采用凝集素作为筛选工具可以快速获得大量与油菜具有特异亲和性的PGPR菌株，菌株N10和K6作为油菜特异性PGPR具有很大的应用潜力。 |
| 关键词: 油菜 植物根际促生菌 亲和性菌株 促生效果 |
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| 基金项目:国际植物营养研究所项目(IPNI-Anhui-19)；2013年安徽省自主创新科技专项“高油酸油菜新品种选育与生产加工关键技术研究及产业化”；安徽省油菜产业技术体系项目(AHCYTX-04)；安徽省自然科学基金项目（1208085QC62）；安徽省高校青年基金重点项目(2013SQRL015ZD) |
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| Screening of affinity rhizosphere bacteria and its growth promotion effect on rape |
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CUI Song-song,BAI Li-min,ZHOU Ke-jin,et al
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| Abstract: |
| 【Objective】Affinity bacteria strains were isolated from rape rhizosphere for obtaining specific rhizobacteria to promote plant growth.【Method】Rhizosphere bacteria were isolated from the rhizosphere of rape using serial dilution method.Rape lectin was prepared and used to rescreen specific affinity strains,which reacted positively with rape lectin.Then their abilities of producing siderophore and indole acetic acid (IAA) as well as activities of amino cyclopropane-1-carboxylic acid (ACC) deaminase and phosphate dissolution were determined.At last,rape seedlings were cultivated in pots with obtained strains to evaluate their growth promoting effects.【Result】A total of 32 strains with specific affinity to rape were obtained,among which 30 had siderophores production ability and 16 reached 5+ level.29 strains had phosphate solubilizing activity and 7 had yields over 100.00 μg/mL.16 strains produced IAA and P17 had the highest yield of 30.48 mg/L.7 strains had ACC deaminase activity.Pot experiment showed that 25 strains had different growth promoting effects,among which strains N10 and K6 possessed significant promotion effects and growth promotion by 22 strains reached the same level as applying full nutrition fertilizers.【Conclusion】Specific affinity plant growth-promoting rhizobacteria strains could be easily screened using lectin as tool.Strain N10 and strain K6 showed excellent application potential. |
| Key words: rape plant growth-promoting rhizobacteria (PGPR) affinity strain growth promoting effect |
