| 摘要: |
| 【目的】比较不同比例配伍的丹参-红花药对对急性血瘀证大鼠血液流变学和血流动力学的影响。【方法】分别制备丹参-红花配比(质量比)为3∶1、2∶1和1∶1的药液（以生药计药物的质量浓度为0.6 g/mL）。取SD雄性大鼠60只，随机分为6组，即丹参-红花3∶1、2∶1、1∶1组和阳性药物对照组、模型对照组、空白组。除空白组外，其余各组大鼠采用皮下注射盐酸肾上腺素0.8 mg/kg和冰水浴复制急性血瘀证模型，然后禁食，自由饮水，12 h后采血，测血液流变学指标，确定造模成功后，对丹参-红花3∶1、2∶1、1∶1组大鼠灌服给药3 g/(kg·d)，阳性药物对照组给予丹红注射液9 mL/(kg·d)，模型对照组和空白组灌服蒸馏水，每日1次，连续8 d。于第8天给药后的30 min，麻醉大鼠，取血，测血液流变学相关指标（全血黏度、血浆黏度、血沉、红细胞压积）和血流动力学指标(左心室收缩压、左室舒张末期压力、左心室最大上升速率、左心室最大下降速率、ST段）。【结果】与空白组比较，模型对照组大鼠全血黏度、血浆黏度、血沉等血液流变学指标均极显著升高，表明急性血瘀证模型构建成功。与模型对照组比较，丹参 红花不同比例配伍均能降低大鼠全血黏度、血浆黏度、血沉，并可抑制缺血导致的大鼠左心室收缩压、左心室最大上升速率和下降速率的降低及左室舒张末期压力、ST段的升高，其中以丹参-红花3∶1配伍效果最好。【结论】丹参-红花药对以3∶1配伍对急性血瘀证大鼠血液流变学和血流动力学有显著的改善作用。 |
| 关键词: 丹参-红花 血瘀证 血液流变学 血流动力学 |
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| 基金项目:国家自然科学基金项目（81573624）；陕西省教育厅科学研究计划项目（2013JK0835） |
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| Impact of Salvia miltiorrhiza-Carthamus tinctorius ratio on hemorheology and hemodynamics of acute blood stasis rats |
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YANG Wen-chao,WANG Xiao-ping,BAI Ji-qing
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| Abstract: |
| 【Objective】This study aimed to comparatively assess the effects of different Salvia miltiorrhiza-Carthamus tinctorius ratios on the hemorheology and hemodynamics of acute blood stasis rats.【Method】The test solutions with S.miltiorrhiza-C.tinctorius ratios of 3∶1,2∶1,and 1∶1 were prepared with the drug concentrations of 0.6 g original medicinal materials per mL.A total of 60 SD rats were randomly divided into blank group,model group as well as S.miltiorrhiza-C.tinctorius 3∶1,2∶1,and 1∶1 groups.Ubcutaneous injection of adrenaline hydrochloride (0.8 mg/kg) and ice water bath were used to establish the acute blood stasis rat model for all groups except the blank group.After 12 h of fasting and free water drinking,blood sample were taken and blood rheology indicators were tested to determine the success of model establishment.Then,S.miltiorrhiza-C.tinctorius groups were given corresponding drugs（3 g/(kg·d)） orally,positive drug control group was given Danhong injection of 9 mL/(kg/d),while model control group and blank group were given distilled water once per day for 8 days.30 min after the treatment on day 8,rats were anesthetized before blood samples were taken to determine blood rheology related indicators (whole blood viscosity,plasma viscosity,blood sedimentation,and red blood cells hematocrit) and hemodynamic indexes (left ventricular systolic blood pressure,left ventricular end diastolic pressure and left ventricular maximum rising rate,left ventricular maximum decline rate,and ST segment).【Result】Compared with the blank group, all hemorheology indexes of model group were significantly increased,indicating that the model was established successfully.Compared with model group,S.miltiorrhiza-C.tinctorius with different ratios reduced the values of whole blood viscosity,plasma viscosity and blood sedimentation,and inhibited left ventricular systolic blood pressure and left ventricular maximum rising rate and falling rate and the left ventricular end diastolic pressure as well as the rise of ST segment of acute blood stasis rats.The S.miltiorrhiza-C.tinctorius with ratio of 3∶1 was the best.【Conclusion】S.miltiorrhiza-C.tinctorius with ratio of 3∶1 could significantly improve hemorheology and hemodynamics of acute blood stasis rats. |
| Key words: Salvia miltiorrhiza-Carthamus tinctorius blood stasis hemorheology hemodynamics |
