| 摘要: |
| 【目的】探讨补充地鳖虫提取物对大鼠运动能力、骨骼肌抗氧化酶表达水平及活性的影响，以明确其作为运动补剂的可能性。【方法】对大负荷游泳训练大鼠按各组平均体质量的0.5,1.0和1.5 g/kg剂量分别补充地鳖虫提取物，在8周大负荷训练结束后测定大鼠力竭游泳时间、骨骼肌抗氧化酶活性及其原因表达水平。【结果】8周大负荷训练后，与单纯大负荷运动组相比，补充地鳖虫提取物组大鼠运动能力显著提高，其骨骼肌SOD、CAT、GST 3种抗氧化酶活性均显著增加，同时3种抗氧化酶编码基因的表达水平也显著上调。【结论】补充地鳖虫提取物能够显著提高大鼠运动能力和骨骼肌抗氧化酶活性，其可能的机理是地鳖虫提取物上调了抗氧化酶编码基因的表达水平。 |
| 关键词: 地鳖虫提取物 运动大鼠 运动能力 骨骼肌抗氧化酶 表达水平 酶活性 |
| DOI： |
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| 基金项目:国家自然科学基金项目（30500391） |
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| Effects of ethanol extract from Eupolyphaga sinensis on sports performance and activities of antioxidant enzymes in skeletal muscle of rats |
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GAO Ying-hui,ZHOU Wan-hong,MA He,et al
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| Abstract: |
| 【Objective】The effects of ethanol extract from Eupolyphaga sinensis (EEES) on sports performance and activities and expression levels of antioxidant enzymes in skeletal muscle of exercised rats were analyzed to investigate the possibility of using it as sports supplement.【Method】The EEES was supplemented to exercised rats at three dose levels of 0.5,1.0 and 1.5 g/kg.The exhaustive swimming time,and activities and encoded genes expression levels of antioxidant enzymes in skeletal muscle were measured after eight weeks training.【Result】The exhaustive swimming time of rats supplemented with EEES increased significantly compared to non-supplemented training rats (α=0.05).The activities of SOD,CAT and GST of EEES supplemented rats were increased significantly compared to non-supplemented training rats.Meanwhile,the expression levels of encoded genes of the three enzymes were up-regulated as well.【Conclusion】The exercise performance and antioxidant enzymes activities in skeletal muscle were improved by EEAC supplementation,which is possibly due to the improvement of encoded gene expression levels by EEAC supplementation in vivo. |
| Key words: ethanol extract of Eupolyphaga sinensis exercised rat exercise performance antioxidant enzymes in skeletal muscle expression level enzyme activity |
