| 摘要: |
| 【目的】解淀粉芽孢杆菌(Bacillus amyloliquefaciens)是从山茶叶片内部分离得到的1株内生拮抗菌株，通过研究该菌株在山茶叶片表面和内部的定殖规律及其对山茶灰斑病的田间防治效果，为新型生防制剂的开发及山茶灰斑病的生物防治提供理论依据。【方法】采用抗生素标记法，结合比较标记菌株和非标记菌株的形态特征、拮抗能力、防病效果，筛选出最佳标记菌株；分别采用喷施和针刺法研究接种浓度、病原菌对生防菌在山茶叶表面及内部定殖动态的影响，并测定生防菌对山茶灰斑病的田间防治效果。【结果】从含500 μg/mL链霉素抗性平板上得到最佳标记菌株，标记菌株与未标记菌株在形态特征、拮抗能力、防病效果方面均无明显差异。定殖试验表明，解淀粉芽孢杆菌能够高效定殖于山茶叶表面及内部，且叶表面的定殖较叶片内部更为高效、持久，其定殖量与接种浓度呈正相关，并在一定时间内维持相当数量，病原菌仅与叶表面定殖密切相关，与生防菌的叶内定殖无关。田间防治试验表明，生防菌解淀粉芽孢杆菌菌悬液对山茶灰斑病有显著生防效果，预先接种生防菌菌悬液后再接种病原菌的生防效果最好，防效为75.35%，先接种病原菌后喷施解淀粉芽孢杆菌菌悬液处理的防效为73.89%，同时接种生防菌菌悬液和病原菌时的防效最低，为72.95%。【结论】生防菌株B.amyloliquefaciens能够有效定殖于山茶叶片表面及内部，且对山茶灰斑病具有显著的生物防治效果。 |
| 关键词: 山茶灰斑病 芽孢杆菌 茶褐斑拟盘多毛孢菌 生物防治 定殖能力 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科技资源共享平台项目(2005DKA21207-13) |
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| Colonization of Bacillus amyloliquefaciens in camellia leaves and its control efficacy against camellia gray spot |
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QIAO Tian-min,ZHANG Jing,RAN Xiao-xiao,et al
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| Abstract: |
| 【Objective】Bacillus amyloliquefaciens is one of the endogenous antagonistic strains separated from the internal camellia leaf.This study investigated the colonization of B.amyloliquefaciens in camellia leaves and its control efficacy on against camellia gray spot to provide references for the development of new bio control agents and biological control of camellia disease.【Method】A mutant strain of B.amyloliquefaciens resistant to streptomycin was screened and obtained with the antibiotic label method,and the contrast tests for morphological characteristics,antagonism abilities,and control effects were conducted.The colonization trials in camellia leaves with different inoculation concentrations and pathogens were determined with the spraying method and needle puncturing method.Field trials were also conducted.【Result】The optimal marked strain was obtained from the resistant plating medium with streptomycin of 500 μg/mL.The morphological characteristics,antagonism abilities,and control effects of the marked strain were not significantly different from the unmarked strains.The colonization trials showed that B.amyloliquefaciens could efficiently colonize on the surface and inside of camellia leaves.In addition,it colonized more efficient and durable on surface of leaves than the inside.The population of colonization was positively correlated with the inoculated concentration,and maintained considerable number at a certain time.The pathogen was related with the surface colonization,but not to inside colonization.The control tests in the field showed that biocontrol strain suspension had significant effect on camellia gray spot.The effect of inoculating pathogen after biocontrol strain was best with the rate of 75.35%.The effect of inoculating pathogen before biocontrol strain was 73.89%.The effect of inoculating biocontrol strain and the pathogen simultaneously was the lowest with rate of 72.95%.【Conclusion】The bio-control strain B.amyloliquefaciens can effectively colonize in both the surface and inside of camellia leaves,and it possessed strong bi-control effects against camellia gray spot. |
| Key words: camellia gray spot Bacillaceae Pestalotiopsis guepini (Desm.)Stey biological control colonizing capacity |
