| 摘要: |
| 【目的】快速高效筛选7型猪链球菌免疫原性蛋白，以作为猪链球菌疫苗的候选分子。【方法】以7型猪链球菌分离株WH07为样品，应用免疫蛋白质组学技术建立了WH07株全菌蛋白的双向电泳体系，并利用蛋白质免疫杂交技术筛选免疫原性蛋白。【结果】获得了分辨率及重复性良好的双向电泳（2-DE）图谱，且得到3个具有免疫反应性的蛋白质点，经质谱鉴定其归属于1个蛋白，即乳酸脱氢酶（LDH）。生物信息学预测表明，该蛋白分子质量为 35 420 ku，理论等电点为5.05，具有2个跨膜区，分别位于第10～28位和第110～129位，并根据预测分析结果成功模拟了蛋白质的三级结构。【结论】从猪链球菌WH07菌株中筛选并鉴定了1个具有免疫原性的蛋白，即乳酸脱氢酶，并模拟了其三级结构。 |
| 关键词: 猪链球菌 免疫相关蛋白 双向电泳 质谱鉴定 蛋白结构预测 |
| DOI： |
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| 基金项目:国家科技重大专项（2012ZX10004214）；国家自然科学基金项目（31060281） |
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| Screening and analysis of immune related proteins of Streptococcus suis serotype 7 |
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GAO Ming-ming,WANG Shu-jie,LIU Yong-gang,et al
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| Abstract: |
| 【Objective】This study aimed to identify the immune-associated proteins of Streptococcus suis serotype 7 as S.suis vaccine candidates.【Method】The two dimensional gel electrophoresis (2-DE) for S.suis proteome separation was established for S.suis WH07 strain and the immune-related proteins were identified by western blot.【Result】Three proteins were recognized by S.suis positive serum and identified as L-lactate dehydrogenase (LDH) by mass spectrometry analysis.The molecular mass of the predicted protein was 35 420 ku,its pI was 5.05,and there were two trans membrane regions at 10-28 and 110-129,respectively.The tertiary structure of the protein was also successfully simulated according to the forecast analysis.【Conclusion】This study screened and identified one immune-related protein from strain WH07 and simulated its tertiary structure. |
| Key words: Streptococcus suis immune related protion two-dimensional gel electrophoresis immunity mass spectrum structure prediction |
