| 摘要: |
| 【目的】研究航天诱变处理对SP1代夏枯草生物学特性和迷迭香酸含量的影响，为夏枯草种质改良和品种选育提供参考。【方法】以神舟八号飞船搭载的夏枯草种子和地面种子(对照)为材料，在夏枯草发育过程中观测记录表型生长指标；在夏枯草收获后，用高效液相色谱法检测其干燥果穗中的迷迭香酸含量，分析航天诱变对夏枯草的影响。【结果】搭载组(SP)夏枯草的株高、主茎粗、分枝粗、果穗粗度、果穗长度、果穗数、地上部分干质量、地上部分鲜质量、果穗干质量、茎叶干质量等表型指标与对照组(CK)相比均有不同程度降低，其中分枝粗、果穗数、果穗粗度、果穗长度、地上部分干鲜质量、果穗干质量、茎叶干质量等指标差异达到极显著水平；搭载组(SP)夏枯草的抽薹期、开花期、盛花期、成熟期均较对照推后；搭载组(SP)干燥果穗中迷迭香酸含量总体较对照组(CK)有所升高，在SP1代中检测到6株含量极高的植株，但总体差异不显著；搭载组SP1代夏枯草生长过程中出现了多种表型变异。【结论】航天诱变增大了夏枯草植株表型的突变率，总体而言对夏枯草表型生长具有抑制作用。 |
| 关键词: 夏枯草 航天诱变 生物学特性 SP1代 迷迭香酸 |
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| 基金项目:陕西省科技统筹项目（2012KTCL02-07） |
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| Effects of space mutation on biological characteristics and rosmarinic acid content of SP1 Prunella vulgaris L. |
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MA Nan,QI Zhi-hong,MAO Ren-jun,et al
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| Abstract: |
| 【Objective】This study investigated the effects of space mutation on biological traits and rosmarinic acid content of Prunella vulgaris L.to provide references for germplasm improvement and variety breeding.【Method】Using the seeds carried by Shenzhou Ⅷ spacecraft and the seeds stayed on earth of Prunella vulgaris L.as materials,growth indexes of phenotype were observed and rosmarinic acid contents in dry ears were measured by high performance liquid after harvesting.Then the differences between these two groups were compared to identify the effects of space mutation on Prunella vulgaris L.【Result】General reductions in plant height,branch width,cluster length,cluster width,cluster number,dry weight of the aerial parts,fresh weight of the aerial parts,dry weight of clusters,and dry weight of stems and leaves were found when comparing the mutagenic treatment group (SP) with the control (CK).The differences in branch width,cluster number,fresh weight of the aerial parts,dry weight of the aerial parts and clusters were extremely significant.The bolting stage,initial blooming stage,full blooming stage,and maturing stage of SP were later than those of CK.The rosmarinic acid content of SP was larger than CK.Six plants with high rosmarinic acid contents were detected in SP1 generation,but the differences were insignificant.Besides,a variety of phenotypic variations were observed in SP1 generation.【Conclusion】Space mutation treatment increased the phenotype mutation rate and inhibited the growth of Prunella vulgaris L. |
| Key words: Prunella vulgaris L. space mutation treatment biological characteristics SP1 generation rosmarinic acid |
