摘要: |
【目的】探究华北落叶松×日本落叶松的种间杂交亲和性以及种子形态的变异,为育种工作和种子园建设提供科学依据。【方法】以6个华北落叶松无性系为母本,5个日本落叶松无性系为父本,通过人工控制授粉获得30个种间杂交组合的球果和杂种种子,并设置母本自由授粉试验作为对照,对种间杂交的结实性状和杂种种子长、宽、种翅长等形态性状进行测定,分析这些性状在杂交组合间及母本自由授粉间的差异。【结果】不同杂交组合间的杂交亲和性差异显著,可分为超亲、良好和较差3类,分别有2、17和11个组合;在华北落叶松×日本落叶松杂交中,种子饱满率和种子效率主要受亲本交互作用的影响,结籽率主要受母本影响;杂种种子大小与母本有关,绝大多数的种翅长度较母本更长,增幅普遍在4%以上,部分组合的增幅达到10%以上。【结论】华北落叶松×日本落叶松种间杂交有重要的育种价值,杂交育种过程中应妥善利用不同亲本组合对杂交亲和性的影响。 |
关键词: 落叶松 种间杂交 亲和性 种子形态 变异 |
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基金项目:河北省林业科技支撑项目(1109402) |
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Variation of crossability and seed shape of Larix principis-rupprechtii and Larix kaempferi interspecific hybrids |
ZHANG Hong-jing,DAI Jian-feng,LIN Yan,et al
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Abstract: |
【Objective】The variation of crossability and seed morphology caused by interspecific hybrid between Larix principis-rupprechtii and Larix kaempferi were studied to provide scientific reference for breeding and establishing seed gardens.【Method】30 crosses were obtained using the control-pollinate method,with parents of 6 L.principis-rupprechtii clones (female) and 5 L.kaempferi clones (male).The maternal free pollinate experiment was set as control.The fruits and seeds of these crosses were collected for testing seed traits and morphological indexes,including seed length,seed width,and seed wing length.The variation of these traits between hybrids and their maternal parents was also analyzed.【Result】The differences of these traits in different hybrid combinations were significant,and all crosses could be classified into 3 categories:over-parent,fine and poor,with 2,17 and 11 combinations,respectively.Seed plumpness and seed efficiency depended on hybridization combination and seed setting was mainly dominated by the female parents.Hybrid seed size was associated with female parent,and the seed wing lengths of most combinations were over 4% longer than their female parents,part of them were even 10%.【Conclusion】The breeding value of Larix principis-rupprechtii×L.kaempferi interspecific hybrids was great,and the effects of different parental combination on crossability should be used efficiently. |
Key words: Larix interspecies cross crossability seed morphology variation |