| 摘要: |
| 【目的】在分子水平上探究苹果属海棠野生种、栽培种及相近属植物之间的亲缘关系，为分类学研究、海棠育种提供参考。【方法】选用4对AFLP引物(M-CTC/E-AGC,M-CTA/E-ACG,M-CAT/E-ACT和M-CAA/E-ACG),分析苹果属海棠9个野生种、8个栽培种以及4个山楂属植物和2个梨属植物的遗传多样性。【结果】1）共扩增出条带114条,其中多态性条带108条,多态位点百分率达94.7%，供试材料表现出丰富的遗传多样性。（2）供试材料在相似系数为0.73时被分为3组，分别为苹果属组、山楂属组和梨属组，因此AFLP分子标记的方法可以将苹果属与梨属、山楂属完全区分开。（3）秦巴山区野生苹果属海棠在相似性系数为0.80时被聚为4组，聚类结果支持Rehder将湖北海棠和垂丝海棠列入山荆子系的分类方法，不支持Langenfelds建立独立的湖北海棠系的分类方法。【结论】基于遗传相似性系数的聚类结果与形态学分类结果基本一致，既验证了形态学分类的正确性，又解决了各分类系统间存在的分歧。 |
| 关键词: 海棠 AFLP 分子标记 亲缘关系 分类 |
| DOI： |
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| 基金项目:国家林业公益性行业科研专项（201204308）；高等学校博士学科点专项科研基金项目（20120204120006） |
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| AFLP analysis on genetic relationship of crabapples |
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XU Man,LI Hou-hua,GUO Yi-bo,et al
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| Abstract: |
| 【Objective】The study investigated the genetic relationship of wild crabapple species, popular cultivars and species of similar genus to provide theoretical support for taxonomic research and breeding new crabapple varieties.【Method】By applying 4 pairs of AFLP (Amplified Fragment Length Polymorphism) primers (M-CTC/E-AGC,M-CTA/E-ACG,M-CAT/E-ACT and M-CAA/E-ACG),9 samples of wild crabapples from Qinling Mountains,8 samples of common crabapple cultivars and 4 Crataegus samples,and 2 Pyrus samples were selected for phylogenetic analysis.【Result】(1) Four primer combinations produced a total of 114 fragments,among which 108 were polymorphic with a polymorphism ratio of 94.7%.(2) With a threshold value of 0.73,the 23 samples were clustered into 3 groups:genus Malus,Crataegus and Pyrus.Molecular markers method was successful to separate genus Malus,Crataegus and Pyrus.(3) With a threshold value of 0.80,the wild species of Qinling Mountains were clustered into 4 groups.The clustering result was in line with traditional morphological classification,which grouped M.hupehensis and M.halliana to Serial Baccatae Rehd instead of a separate Ser.Hupehenses Langenf. 【Conclusion】Clustering based on genetic similarity coefficients was basically in line with morphological classification,which not only verified the correctness of the morphological classification,but also solved the existing differences between various classification systems. |
| Key words: crabapples AFLP molecular markers relationship classification |
