| 摘要: |
| 【目的】比较蜡蚧轮枝菌V3450菌株对榕管蓟马及斯氏钝绥螨的毒力差异，为利用天敌昆虫和虫生真菌协同控制榕管蓟马等害虫提供技术支持。【方法】室内测定榕管蓟马成虫和斯氏钝绥螨雌成螨受蜡蚧轮枝菌V3450菌株侵染后的死亡趋势，构建时间-剂量-死亡率（TDM）模型，比较供试菌株对榕管蓟马成虫及斯氏钝绥螨雌成螨的LC50和LT50。【结果】蜡蚧轮枝菌V3450菌株侵染后，榕管蓟马和斯氏钝绥螨的死亡趋势均随菌剂浓度的升高及侵染时间的延长而上升，但榕管蓟马的累计校正死亡率及死亡趋势的变幅均明显高于斯氏钝绥螨，其中1.00×107和1.00×108 mL-1蜡蚧轮枝菌V3450菌株侵染10 d，斯氏钝绥螨雌成螨的累计校正死亡率分别仅是榕管蓟马成虫的13.30%和 27.94%。构建的榕管蓟马和斯氏钝绥螨TDM模型均能通过Pearson χ2和Hosmer-Lemeshow检验，拟合结果与试验观察结果相吻合，能够无偏地描述蜡蚧轮枝菌V3450菌株对榕管蓟马成虫和斯氏钝绥螨雌成螨的时间-剂量互作效应。蜡蚧轮枝菌V3450菌株对榕管蓟马的剂量和时间效应均明显强于斯氏钝绥螨，5个不同浓度蜡蚧轮枝菌V3450菌株对斯氏钝绥螨雌成螨的LT50均超出TDM模型估测范围，斯氏钝绥螨雌成螨受侵染3 d后的LC50是同一侵染时间榕管蓟马成虫LC50的2.51×102～1.29×105倍。【结论】蜡蚧轮枝菌V3450菌株对榕管蓟马的毒力较强，而对斯氏钝绥螨的毒力较弱，榕管蓟马对供试菌株时间-剂量互作效应的响应要远强于斯氏钝绥螨。 |
| 关键词: 蜡蚧轮枝菌 榕管蓟马 斯氏钝绥螨 时间 剂量 死亡率（TDM）模型 毒力 |
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| 基金项目:福建省农业科技重大专项专题(2013NZ0002-4)；福建省属公益优势领域重点项目(2014R1024-1)；福建省自然科学基金项目(2014J01110)；福建省农业科学院科技创新团队项目(CXTD-2-1313) |
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| Toxicity comparison of Lecanicillium lecanii V3450 strain to Gynaikothrips uzeli and Amblyseius (Typhlodromips) swirskii |
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YU De-yi,HUANG Peng,YAO Jin-ai,et al
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| Abstract: |
| 【Objective】This study compared the difference in toxicity of Lecanicillium lecanii V3450 strain against Gynaikothrips uzeli and Amblyseius (Typhlodromips) swirskii to provide technical reference for cooperative control of G.uzeli and other pests using natural enemy insects and entomogenous fungus.【Method】The mortality trends of G.uzeli adults and A.swirskii female adults infected by L.lecanii V3450 strain were investigated in laboratory,the time-dose-mortality (TDM) model of L.lecanii V3450 strain against G.uzeli adults and A.swirskii female adults constructed,and their LT50 and LT50 were compared.【Result】The mortality trends of G.uzeli adults and A.swirskii female adults increased as the increase of concentration and infection time of L.lecanii V3450 strain.The corrected accumulative mortality and mortality trend amplitude of G.uzeli adults were significantly higher than those of A.swirskii female adults.Ten days after inoculated-pathogen with concentrations of 1.00×107 and 1.00×107 conidia/mL,the corrected accumulative mortalities of A.swirskii female adults were 13.30% and 27.94% of G.uzeli adults,respectively.The TDM model of L.lecanii V3450 strain to G.uzeli adults and A.swirskii female adults passed Pearson’s chi-square test and Hosmer-Lemeshow test.The TDM results were coincided with experimental observation,and accurately reflected the time-dose relationships of L.lecanii V3450 strain to G.uzeli adults and A.swirskii female adults.The time-dose effect of L.lecanii V3450 strain to G.uzeli adults was stronger than to A.swirskii female adults.The LT50 of L.lecanii V3450 strain to A.swirskii female adults at five concentrations were not in estimation range of TDM model.Three days after inoculated-pathogen,the LT50 of L.lecanii V3450 strain to A.swirskii female adults were 2.51×102-1.29×105 times higher than to G.uzeli adults.【Conclusion】The toxicity of L.lecanii V3450 strain was stronger to G.uzeli than to A.swirskii.Comparing with A.swirskii,the response of G.uzeli to time-dose effect of L.lecanii V3450 strain was stronger. |
| Key words: Lecanicillium lecanii Gynaikothrips uzeli Amblyseius (Typhlodromips) swirskii time-dose-mortality (TDM) model toxicity |
