| 摘要: |
| 【目的】探索渗透性冷冻保护剂甘油、乙二醇、二甲基亚砜(DMSO)、二甲基乙酰胺(DMA)对山羊精子的冷冻保护效果。【方法】采用假阴道法采集繁殖性能正常、健康、年龄2～4岁的6只西农萨能奶山羊种公羊精液，以Tris-卵黄为基础稀释液，分别添加体积分数为6%的甘油、3%和6%的乙二醇、3%和6%的DMSO、3%和6%的DMA，测定冷冻 解冻后精子活率、顶体完整性、质膜完整率、线粒体完整性、谷胱甘肽还原酶活性和丙二醛（MDA）浓度，对比不同冷冻保护剂对山羊精子的冷冻保护效果。【结果】甘油(6%)和乙二醇(3%，6%)组解冻后精子活率均极显著(P＜0.01)高于DMSO(3%，6%)和DMA(3%，6%)处理组；甘油(6%)、DMA(6%)和乙二醇(3%)组的线粒体完整性极显著(P＜0.01)高于其他组；甘油(6%)和乙二醇(3%，6%)组的质膜完整率极显著(P＜0.01)高于其他组；从顶体完整性、谷胱甘肽还原酶活性和MDA浓度等指标看，甘油(6%)和乙二醇(3%，6%)组极显著(P＜0.01)优于其他组。【结论】稀释液中添加乙二醇(3%，6%)和甘油(6%)都能明显提高山羊冷冻精子品质，其中3%乙二醇可以替代甘油作为山羊精液冷冻保护剂从而发挥更好的抗冷冻保护作用。 |
| 关键词: 山羊 冷冻保护剂 精液 冷冻保存 |
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| 基金项目:国家公益性行业（农业）科研专项（201103038）；陕西省重大科技创新项目（2009ZKC07-01-1） |
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| Effects of penetrating cryoprotectants supplementation on semen quality and oxidative stress variables in frozen thawed dairy goat semen |
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XI Li-meng,LUO Jun,YANG Di-kun,et al
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| Abstract: |
| 【Objective】This study explored the effects of penetrating cryoprotectants including glycerol,ethylene glycerol,dimethyl sulfoxide (DMSO) and dimethyl acetamide (DMA) supplementations on freezing goat spermatozoa.【Method】Semen samples were collected from 6 healthy,2-4 years old Xinong Saanen dairy goats by artificial vagina method.Semen samples were diluted in Tris-egg yolk with glycerol (6%),ethylene glycerol (3%,6%),DMSO (3%,6%) and DMA (3%,6%) before being frozen at -196 ℃.Then,the effects on semen quality and oxidative stress variables in frozen-thawed dairy goat semen were evaluated through assessing motility,acrosome integrity,plasma membrane integrity,mitochondrial activity,and concentrations of GSH and MDA.【Result】After thawing,sperm motilities with glycerol (6%) and ethylene glycerol (3%,6%) were greater (P＜0.01) than those with DMSO (3%,6%) and DMA (3%,6%).Mitochondrial activities with glycerol (6%) and ethylene glycerol (3%) and DMA(6%) were greater (P＜0.01) than others.Plasma membrane integrities with glycerol (6%) and ethylene glycerol (3%,6%) were greater (P＜0.01) than others.Acrosome integrity,GSH concentration and MDA concentration with ethylene glycerol (3%) were greater (P＜0.01) than others.【Conclusion】Both glycerol (6%) and ethylene glycerol (3%,6%) could protect goat sperm against freezing.As cryoprotectant supplementation,ethylene glycerol 3% can provide greater protection for goat semen than glycerin |
| Key words: goat cryoprotectant samen cryopreservation |
