| 摘要: |
| 【目的】用白痢鸡粪培养蝇蛆，探究蝇蛆抗菌肽对鸡白痢沙门菌感染雏鸡的治疗效果。【方法】用鸡白痢沙门菌感染雏鸡，收集雏鸡粪便培养蝇蛆，粗提蝇蛆抗菌肽，测定其含量和抑菌活性，试验同时设健康鸡粪便培养的蝇蛆抗菌肽作为对照。分别用白痢鸡粪和健康鸡粪培养的蝇蛆抗菌肽治疗白痢病鸡，测定其肠道细菌数量和治愈率，试验同时设抗生素组（恩诺沙星）和未治疗对照组。【结果】白痢鸡粪培养的蝇蛆抗菌肽含量显著高于健康鸡粪培养的蝇蛆抗菌肽(P<0.05)；2种鸡粪培养的蝇蛆抗菌肽对大肠埃希菌、沙门菌和葡萄球菌均有抑制作用，但白痢鸡粪培养的蝇蛆抗菌肽的抑菌活性显著高于健康鸡粪培养的蝇蛆抗菌肽(P<0.05)；蝇蛆抗菌肽治疗白痢病鸡后，白痢鸡肠道细菌数量显著低于对照组(未治疗组)(P<0.05)，稍高于抗生素治疗组(P>0.05)，但白痢鸡肠道细菌数量均达到健康鸡的标准范围。白痢鸡粪培养蝇蛆抗菌肽组的治愈率为43.33%，与健康鸡粪培养蝇蛆抗菌肽组和抗生素组差异不明显。【结论】白痢鸡粪培养的蝇蛆抗菌肽对沙门菌感染的鸡白痢病具有治疗作用。 |
| 关键词: 白痢鸡粪 蝇蛆抗菌肽 抑菌活性 治愈率 |
| DOI： |
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| 基金项目:国家自然科学基金项目（31060281）；兵团科技成果转化及产业化引导计划项目（2012BD056） |
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| Curing effects of maggot antimicrobial peptides cultured with pullorum chicken feces against Salmonella pullorum infected chicken |
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LIU Yu,ZAN Ha-er,HE Xiao-hui,et al
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| Abstract: |
| 【Objective】Maggot antimicrobial peptides (AMPs) cultured with pullorum chicken feces were used to cure Salmonella pullorum infected chicken.【Method】In this research,maggot cultured by pullorum disease of chicken feces were collected,and concentration and antibacterial activity of AMPs were measured after AMPs were extracted crudely.Maggot cultured with uninfected healthy chickens was used as control.Then,maggot AMPs were used to cure Salmonella pullorum infected chicken,and the number of intestinal bacteria and cure rate were measured.One antibiotic group and one untreated group were also set up.【Result】The concentration and antibacterial activity of maggot AMPs cultured with pullorum chicken were significantly higher than those of the healthy group (P<0.05).AMPS from both groups had inhibition ability against E.coli,Salmonella and Staphylococcus aureus.The number of intestinal bacterial in infected chicken was significantly lower than that in the control group (untreated group) (P<0.05),but higher than that in the antibiotic treatment group (P<0.05).It met the standard of intestinal number for healthy chicken.The cure rate of maggot AMPs cultured by pullorum chicken was 43.33%,the difference is not obvious with the chicken manure and health training bots antibacterial peptide group.【Conclusion】Maggot AMPs cultured with pullorum chicken feces had curing effects against Salmonella pullorum infected chicken. |
| Key words: white diarrhea chicken maggot antimicrobial peptides antibacterial activity cure rate |
