| 摘要: |
| 【目的】研究日粮钙水平对仔猪血清碱性磷酸酶活性、骨钙素质量浓度、血清钙浓度及胫骨钙含量和密度的影响，为钙调控仔猪成骨细胞活性提供依据。【方法】采用单因子完全随机分组设计，将60头杜长大仔猪（体质量（21.8±2.1） kg）分成6个处理，每处理设10个重复，每重复1头猪。各处理分别饲喂钙水平为0.30%，0.45%，0.60%，0.75%，0.90%和1.05%的玉米 豆粕型日粮。预饲期为7 d，正式试验期28 d，并在正式试验的14和28 d屠宰仔猪，测定其血清钙浓度、血清活性标志物碱性磷酸酶活性和骨钙素质量浓度及胫骨钙含量和密度，分析钙水平对仔猪成骨细胞活性和胫骨性能的影响。【结果】随着日粮钙水平的提高，血清碱性磷酸酶活性呈先减小后增大的趋势(P<0.05)，且在日粮钙水平为0.75%时出现最小值；血清骨钙素质量浓度呈先增大后减小的趋势(P<0.05)，且在钙水平为0.75%时达到最大(P<0.05)；日粮钙水平对血清钙浓度无显著影响(P>0.05)，但对胫骨钙含量、脱脂胫骨干质量、胫骨灰分含量、胫骨密度4项胫骨性能指标影响显著(P<0.05)，且在日粮钙水平为0.75%时，各项胫骨性能指标均达到最大。【结论】日粮钙水平为0.75%时，仔猪胫骨中成骨细胞的矿化作用最大，增殖活性最小。 |
| 关键词: 仔猪 日粮钙水平 成骨细胞活性 血清钙 胫骨性能 |
| DOI： |
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| 基金项目:国家公益性行业（农业）科研专项（200903006） |
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| Effects of dietary calcium level on osteoblast activity and tibia performance of piglets |
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ZOU Lei,JI Hong-qin,LIU Yu-han,et al
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| Abstract: |
| 【Objective】The effects of different calcium levels on calcium content in bones,serum alkaline phosphatase concentration,serum osteocalcin concentration,serum calcium concentration,calcium content in tibia and BMD of tibia of piglets were investigated to provide theoretical basis for using calcium levels in dietary to adjust osteoblast activity of piglets.【Method】The completely randomized design was adopted and a total of 60 experimental piglets with similar body weights (21.8±2.1) kg were randomly allotted into 6 treatments with 10 repetitions in each treatment and one pig was a repetition.Piglets were fed diets with six different calcium levels (0.30%,0.45%,0.60%,0.75%,0.90% and 1.05%) randomly in a single factorial arrangement.The preparation trial period was 7 d,and the trial period was 28 d.The serum alkaline phosphatase concentration,serum osteocalcin concentration,serum calcium concentration and tibia performance were measured at d14 and d28 to investigate the effects of calcium level on ossification of osteoblasts and tibia performance.【Result】Alkaline phosphatase concentration in serum decreased at first and increased subsequently with the increase of dietary calcium level,and the lowest value was observed in 0.75% group (P<0.05).Osteocalcin concentration in serum was contrary to alkaline phosphatase,and it reached the peak in 0.75% group (P<0.05).Calcium content in serum of piglets had no significant difference with the increase of dietary calcium (P>0.05),but the effects on calcium content of tibia,dry weight of defatted tibia,ash content in defatted tibia and BMD of tibia were significant (P<0.05) withthe highest values in 0.75% group (P<0.05). 【Conclusion】When the calcium level was 0.75%,ossification of osteoblasts in tibia was the greatest and the activity was the minimum. |
| Key words: piglets calcium level of diet osteoblast activity serum calcium concentration tibia performance |
