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神经激肽B在大鼠下丘脑-垂体-卵巢轴上的表达
宋 爽1, 崔 培1, 王尧尧,等1
安徽农业大学 动物科技学院
摘要:
【目的】探究初情期启动前、启动中、启动后大鼠下丘脑、垂体和卵巢中神经激肽B(Neurokinin B,NKB)的表达变化。【方法】以SD雌性大鼠为研究对象,分别取大鼠的下丘脑、垂体和卵巢组织制作冰冻切片,运用免疫荧光染色法检测SD大鼠下丘脑、垂体和卵巢上NKB的分布。【结果】NKB主要分布于各时期大鼠下丘脑的弓状核、腹内侧核、室旁核、腺垂体以及卵巢的黄体细胞、间质细胞。但NKB在不同时期表达水平不同,在初情期启动前(出生后25 d)和启动后(出生后60 d)表达最强,在初情期启动中最弱(P<0.05)。同一时期不同核团之间NKB表达水平也不同,初情期启动前和启动中室旁核最强,弓状核次之,腹内侧核最弱(P<0.05);在初情期启动后,弓状核和腹内侧核中NKB的表达显著高于室旁核(P<0.05)。腺垂体中NKB在初情期启动时表达最强,初情期启动前次之,初情期启动后最弱(P<0.05)。卵巢间质细胞中NKB在初情期启动中的表达显著低于初情期启动前、后(P<0.05)。卵巢黄体细胞中NKB在初情期启动后表达最强,初情期启动时最弱。【结论】NKB可能与初情期启动的调控密切相关。
关键词:  神经激肽B  初情期启动  大鼠  下丘脑  垂体  卵巢
DOI:
分类号:
基金项目:高等学校博士学科点专项科研基金博导类资助课题(20123418110004);安徽省自然科学基金项目(1208085MC39)
Expression of Neurokinin B in hypothalamus,pituitary and ovary of rats
SONG Shuang,CUI Pei,WANG Yao-yao,et al
Abstract:
【Objective】This study aimed to investigate the change in expression of Neurokinin B (NKB) in hypothalamus,pituitary and ovary of rats at prepuberty,puberty onset and post puberty.【Method】 Females SD rats were selected and their hypothalamus,pituitary and ovary were taken for frozen section before immunofluorescence staining technology was used to detect the distribution of NKB in hypothalamus,pituitary and ovary.【Result】NKB was mainly distributed in arcuate nucleus,ventromedial hypothalamic nucleus,paraventricular nucleus of the hypothalamic,adenohypophysis cells,and corpus luteum cells and stromal cells of ovary of rats.Expression intensity of NKB in hypothalamu was different at different periods with the strongest expression at prepuberty (on postnatal 25th day) and post puberty (on postnatal 60th day) (P<0.05) and the weakest at puberty-onset (P<0.05).Different nucles had different expression levels at the same time,at prepuberty and puberty onset the strongest expression was found in the paraventricular nucleus followed by arcuate nucleus,while the weakest was in the ventromedial hypothalamic nucleus (P<0.05);while at post puberty,the strongest expression was found in arcuate nucleus and ventromedial hypothalamic nucleus,while the weakest expression was in the paraventricular nucleus.The strongest expression intensity of NKB in pituitary was found at puberty-onset,followed by prepuberty and post puberty (P<0.05).Expression intensity of NKB in stromal cells of ovary at puberty onset was significantly lower than that at prepuberty and post puberty (P<0.05).The weakest expression intensity of NKB was found at puberty-onset,while the strongest expression was at post puberty in stromal cells of ovary.【Conclusion】NKB may be closely related to the control of puberty onset.
Key words:  NKB  puberty onset  rats  hypothalamus  pituitary  ovary