| 摘要: |
| 【目的】对金娃娃萱草（Hemerocallis hybridus Stella de Oro）和大花萱草(Hemerocallis middendorffii)在Cu-Cd复合胁迫条件下的生长及生理特性进行研究,为重金属污染土壤的花卉植物修复提供理论依据。【方法】以金娃娃萱草和大花萱草多年生分株苗为材料,进行盆栽试验，以不处理植株为对照，探讨不同Cu-Cd复合处理(T1：Cu 100 mg/kg＋Cd 0.3 mg/kg，T2：Cu 400 mg/kg＋Cd 1 mg/kg，T3：Cu 800 mg/kg＋Cd 20 mg/kg，T4：Cu 1 200 mg/kg＋Cd 100 mg/kg)和胁迫时间(30，60，90,120 d)下,2种萱草株高、干质量、叶片光合色素含量、净光合速率、蒸腾速率及抗氧化酶（POD、SOD）活性等生理指标的变化。【结果】T1处理促进了大花萱草的生长，随着Cu-Cd胁迫的继续加剧，其植株高度和生物量下降；与对照相比，Cu-Cd胁迫处理金娃娃萱草植株高度和生物量一直呈下降趋势。与对照相比，大花萱草T1处理SOD活性有所增加，而后随胁迫强度的增加，SOD活性逐渐下降；金娃娃萱草SOD活性一直呈下降趋势；2种萱草POD活性均随Cu-Cd复合含量的增加逐渐上升。金娃娃萱草叶片叶绿素含量随Cu-Cd复合含量的增加而降低，而大花萱草叶片叶绿素含量呈先增加后降低趋势；2种萱草净光合速率和蒸腾速率均呈双峰曲线，峰值出现在10: 00和16: 00。【结论】大花萱草对Cu-Cd复合胁迫的耐受性较金娃娃萱草强，更有利于对重金属污染土壤的修复。 |
| 关键词: 萱草 Cu-Cd复合胁迫 抗氧化酶 土壤修复 |
| DOI： |
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| 基金项目:吉林省科学技术厅项目“长白山特种经济植物保护及配套关键技术研究”（20100259） |
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| Effects of Cu-Cd combined pollution on growth and antioxidant enzyme activity of 2 Hemerocallis fulva varieties |
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GUAN Meng-xi,ZHOU Xu-dan,DONG Ran
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| Abstract: |
| 【Objective】The study investigated the growth and physiological characteristics of Hemerocallis hybridus ‘Stella de Oro’ and Hemerocallis middendorffii under Cu-Cd combined stress to provide theory basis for phytoremediation of heavy metal contaminated soils.【Method】 Perennial divided seedlings of Hemerocallis hybridus ‘Stella de Oro’ and Hemerocallis middendorffii were planted in pots to investigate the changes in plant height,dry weight,leaf,photosynthetic rate and transpiration rate and anti-oxidase activity （POD and SOD) and other physiological indexes under different Cu-Cd combined concentrations (T1:Cu 100 mg/kg＋Cd 0.3 mg/kg,T2：Cu 400 mg/kg＋Cd 1 mg/kg,T3:Cu 800 mg/kg＋Cd 20 mg/kg,and T4:Cu 1 200 mg/kg＋Cd 100 mg/kg) and stress times (30,60,90,and 120 d).【Result】T1 promoted the growth of Hemerocallis middendorfii,and the height and dry weight decreased as the increase of Cu-Cd stress.The height and dry weight of Hemerocallis hybridus ‘Stella de Oro’ decreased continuously.T1 increased SOD activity of Hemerocallis middendorfii,and it decreased with the increase of stress.SOD activity of Hemerocallis hybridus ‘Stella de Oro’ decreased continuously.POD activities of both varieties increased as the increase of Cu-Cd content.The chlorophyll content of Hemerocallis hybridus ‘Stella de Oro’ decreased with the increase of Cu-Cd content,while that of Hemerocallis middendorfii firstly increased and then decreased.Photosynthetic rates and transpiration rates of both varieties had the shape of bimodal with peaks at 10: 00 am and 16: 00 pm.【Conclusion】Hemerocallis middendorfii had better tolerance against Cu-Cd combined stress than Hemerocallis hybridus ‘Stella de Oro’,and it was more conducive to remediation of heavy metal polluted soil. |
| Key words: Hemerocallis fulva Cu-Cd combined stress anti-oxidation enzyme soil remediation |
