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高丹草10个重要性状的QTL定位分析
房永雨1, 于肖夏1, 于 卓,等1
内蒙古农业大学 农学院
摘要:
【目的】确定高丹草茎叶比、分蘖数等9个重要农艺性状及氢氰酸含量的QTL位点,为深入开展高丹草分子标记辅助育种等研究提供依据。【方法】以散穗高粱、红壳苏丹草及高丹草F2群体为材料,在已构建的高丹草高密度分子连锁遗传图谱上,采用多MQM模型法对氢氰酸含量、株高、茎粗、叶片数、叶长、叶宽、茎叶比、穗长、单株干质量、分蘖数10个性状进行QTL定位分析。【结果】高丹草F2群体的10个性状测量值呈正态分布,均可用于QTL分析;控制10个性状的QTL有24个,分布在除连锁群LGⅨ外的其余9个连锁群上,遗传贡献率变幅为6.1%~43.6%。在24个QTL中,控制氢氰酸含量的QTL有2个,控制叶片数、分蘖数和单株干质量的 QTL各有1个,控制茎粗、叶长、叶宽、穗长、茎叶比的QTL各有3个,控制株高的QTL有4个。【结论】在已构建的高丹草高密度分子连锁遗传图谱上,定位出氢氰酸含量及株高、茎粗等10个性状的24个QTL。
关键词:  高丹草  杂种F2群体  重要性状  QTL定位
DOI:
分类号:
基金项目:国家“973”计划项目(2014CB138703);国家自然科学基金面上项目(31472141)
QTL mapping analysis of 10 important traits in Sorghum Sudangrass hybri
FANG Yong-yu,YU Xiao-xia,YU Zhuo,et al
Abstract:
【Objective】This study identified the QTLs of important agronomic traits of Sorghum-Sudangrass such as hydrocyanic acid content,stem-leaf ratio and tiller number to provide the basis for the further study of molecular assisted breeding of Sorghum-Sudangrass.【Method】Sorghum bicolor,Sorghum sudanense and Sorghum-Sudangrass hybrid F2 population were selected as materials.Based on the constructed high density genetic linkage map of Sorghum-Sudangrass,the QTLs of 10 main traits including hydrocyanic acid content,plant height,stem diameter,leaf number,leaf length,leaf width,stem-leaf ratio,spikelet length,dry weight per plant,and tiller number were identified and analyzed using MQM QTL models.【Result】The measurements of 10 traits of Sorghum-Sudangrass hybrid F2 population showed a normal distribution,indicating that they could be used for QTL analysis.A total of 24 QTLs were identified and they distributed on 9 linkage groups except LGⅨ,with the contributions of 6.1% to 43.6%.Of all the 24 QTLs,the numbers of QTLs controlling hydrocyanic acid content,leaf number,tiller number and dry weight per plant,stem diameter,leaf length,leaf width,panicle length and stem-leaf ratio,and plant height were 2,1,1,1,3,3,3,3,3,and 4,respectively.【Conclusion】A total of 24 QTLs were located for 10 important traits of Sorghum-Sudangrass on the constructed high-density molecular genetic maps.
Key words:  sorghum-sudangrass hybrid  hybrid F2 population  important traits  QTL location