| 摘要: |
| 【目的】建立‘雪球’海棠离体植株组织培养快繁体系，并对组织培养体系进行优化。【方法】以‘雪球’海棠当年生茎段为起始接种材料，获得无菌苗，利用无菌苗和试管苗叶片为材料，以MS为基础培养基，采用正交试验研究不同激素及其配比对雪球组培苗扩繁、植株再生和组培苗生根的影响。【结果】用2 g/L HgCl2处理‘雪球’海棠茎段外植体是较为恰当的消毒及获得无菌苗的方法。在MS+2.5 mg/L 6-BA+0.3 mg/L IBA培养基中组培苗的总增殖倍数最高，为9.11，为最适增殖培养基；在MS+0.5 mg/L 6-BA+0.3 mg/L IBA培养基中组培苗的总增殖倍数为6.38，分化的芽生长良好，无玻璃化，为获取叶盘不定芽再生试验用叶片的最适培养基。在MS+0.2 mg/L TDZ+0.1 mg/L NAA培养基（以山梨醇为碳源）中，叶盘不定芽再生效率最高，为60.71%，为不定芽再生最适培养基。‘雪球’海棠生根容易，在1/2 MS+0.2 mg/L IBA培养基中，组培苗培养7 d开始生根，生根率为100.0%。【结论】建立了‘雪球’海棠组织培养体系，明显提高了其繁殖速率，使其生根率可达到100.0%。 |
| 关键词: ‘雪球’海棠 组织培养 植株再生 生根诱导 |
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| 基金项目:国家林业公益性行业科研专项（201204308）；高等学校博士学科点专项（20120204120006） |
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| Establishment and optimization of tissue culture system for Malus ‘Snow drift’ |
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WANG Bing-jie,LI Hou-hua,QUE Yi,et al
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| Abstract: |
| 【Objective】This study aimed to establish and optimize a tissue culture system for Malus‘Snow drift’.【Method】Stems with buds of Malus ‘Snow drift’ were used as explants to obtain aseptic seedlings.Using aseptic seedlings and leaves as materials and MS as basal medium,orthogonal test was conducted to analyze the effects of hormone on propagation,shoots regeneration and rooting of tissue cultured seedlings.【Result】The treatment with 2 g/L HgCl2 was efficient for disinfecting and obtaining aseptic seedlings.The optimum medium for shoot proliferation was MS+2.5 mg/L 6-BA+0.3 mg/L IBA with the average proliferation of 9.11.The optimum medium for healthy leaves was MS+0.5 mg/L 6-BA+0.3 mg/L IBA with the average proliferation of 6.38,and the health index was highest.The optimum medium for adventitious shoot regeneration was MS+0.2 mg/L TDZ+0.1 mg/L NAA and using sorbitol as carbon source,with the highest regeneration rate of 60.71%.The optimum medium for rooting was 1/2 MS+0.2 mg/L IBA,with the rooting rate of 100.0% after 7 d.【Conclusion】The established tissue culture system for Malus ‘Snow drift’ improved the reproduction rate significantly with the rooting rate of up to 100.0%. |
| Key words: Malus‘Snow drift’ tissue culture shoots regeneration root induction |
