| 摘要: |
| 【目的】分析5-氨基乙酰丙酸（ALA）处理对苹果幼果黄酮含量及查尔酮合成酶基因（CHS）、查尔酮异构酶基因（CHI）表达量的影响，以确定适宜的ALA处理质量浓度和处理时间。【方法】在苹果疏果期前，用0（对照），100，200，300和400 mg/L ALA处理苹果幼果，采用紫外分光光度法测定ALA处理后3，6，9，12 d幼果中的黄酮含量，同时利用荧光定量法测定幼果中CHS和CHI基因的相对表达量。【结果】在ALA质量浓度为0～300 mg/L 时，随着ALA质量浓度的升高，苹果黄酮含量与CHS、CHI基因表达量均相应升高；在ALA质量浓度升至400 mg/L时，各项指标均表现出下降趋势。用不同质量浓度ALA处理苹果幼果后，幼果的黄酮含量和CHS、CHI基因表达量均较对照明显提高，其中黄酮含量在处理后12 d达到最高，而CHS和CHI基因相对表达量在处理9 d时达到最高，12 d后开始下降。【结论】为提高苹果疏除果中的黄酮含量，宜选择300 mg/L的ALA在疏果前6~9 d对苹果幼果进行喷洒。 |
| 关键词: 苹果幼果 黄酮 CHS CHI 基因表达 |
| DOI： |
| 分类号: |
| 基金项目:现代农业产业技术体系建设专项（GK661001） |
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| Effect of 5-Aminolevulinic acid on flavonoids content and expression of CHS and CHI genes of young apples |
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CHEN Lei,GUO Yu-rong,ZHANG Xiao-rui,et al
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| Abstract: |
| 【Objective】The study aimed to explore the effect of 5 aminolevulinic acid (ALA) on flavonoids content and relative expression of chalcone sythase (CHS) and chalcone isomerase (CHI) of young apples to determine suitable ALA mass concentration and processing time.【Method】Before thinning young apples,ALA solutions with concentrations of 0 (CK),100,200,300 and 400 mg/L ALA were used to treat young apples and contents of young apple flavonoids were determined by UV.At the same time,the relative expression of CHS and CHI was determined by qPCR.【Result】When the ALA mass concentration was 0-300 mg/L,the content of flavonoids and the relative expression of CHS and CHI increased as the increase of ALA mass concentration.When the mass concentration of ALA increased to 400 mg/L,all indexes started to decrease.The content of flavonoids and the relative expression of CHS and CHI were significantly improved by ALA solutions with different mass concentrations.Among them,the content of flavonoids achieved the highest values at 12 d,and the maximum relative expression of CHS and CHI values were achieved at 9 d and began to fall after 12 d.【Conclusion】To improve the content of flavonoids in thinned young apples,300 mg/L ALA was suitable to be sprayed 6-9 d before thinning. |
| Key words: young apple flavonoids CHS CHI gene expression |
