| 摘要: |
| 【目的】探讨铅胁迫对黑麦草富集特性及生理特性的影响，为铅污染土壤的植物修复提供依据。【方法】以黑麦草为试验材料，采用盆栽试验，研究土壤中不同含量(0,500,1 000,2 000,3 000 mg/kg)铅胁迫下黑麦草对铅的吸收、富集规律及铅对黑麦草叶绿素、丙二醛、可溶性蛋白含量和SOD、POD、CAT活性的影响。【结果】当土壤中铅含量为500～1 000 mg/kg时，黑麦草地上部干质量明显高于其他处理；而当土壤铅含量为2 000～3 000 mg/kg时，黑麦草地上部干质量明显降低。随着土壤铅含量的增加，黑麦草根、茎、叶中的铅含量均明显增加，呈明显的剂量效应关系；黑麦草叶片的叶绿素a、叶绿素b和总叶绿素含量均呈下降趋势，可溶性蛋白含量呈上升趋势，丙二醛及SOD、POD、CAT活性呈现明显的先升后降趋势。【结论】不同铅胁迫条件下，黑麦草对铅的富集特性和生理特征有明显变化，黑麦草适用于铅含量低于1 000 mg/kg污染土壤的植物修复。 |
| 关键词: 黑麦草 铅胁迫 植物修复 富集特性 生理指标 抗氧化酶 |
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| 基金项目:国家自然科学基金项目(31270665)；陕西省矿产资源勘查与综合利用重点实验开放课题基金项目（2014HB003）；长安大学“国家级大学生创新创业训练计划”项目(201210710116) |
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| Effects of lead stress on accumulation capacity and physiological metabolism of ryegrass |
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YANG Ming-yan,LIANG Yu-yan,ZENG De-bang,et al
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| Abstract: |
| 【Objective】This study explored the effects of lead stress on the accumulation capacity and physiological metabolism of ryegrass to improve the remediation of lead contaminated soil.【Method】The effects of different lead contents (0,500,1 000,2 000,and 3 000 mg/kg) on accumulation capacity of lead,contents of chlorophyll,MDA,and soluble proteins and activities of SOD,POD and CAT of ryegrass were studied by pot experiment.【Result】Aboveground dry weights of ryegrass were significantly higher than other treatments when the lead contents were 500-1 000 mg/kg soil,while they were significantly lower when lead contents were 2 000-3 000 mg/kg soil.With the increase of soil lead content,lead contents in different parts of ryegrass increased with a dose-dependent manner.The contents of chlorophyll a and b decreased,the contents of MDA and soluble proteins increased,and the activities of SOD,POD and CAT increased initially before decreasing.【Conclusion The accumulating capacity and physiological metabolism of ryegrass changed with different lead stresses.Ryegrass was suitable for phytoremediation of soil with lead concentration of less than 1 000 mg/kg soil. |
| Key words: ryegrass lead stress phytore mediation accumulation capacity physiological metabolism antioxidant enzyme |
