| 摘要: |
| 【目的】克隆并分析高原牦牛Toll样受体1基因（TLR1）的特点。【方法】提取麦洼牦牛脾脏RNA，反转录合成cDNA，以其为模板分段扩增后拼接获得麦洼牦牛TLR1基因编码区序列，采用相关分析软件对基因进行序列分析，并对其编码的蛋白质进行基本理化性质分析及预测。【结果】分段扩增获得了TLR1基因1 484 bp的上游序列和823 bp的下游序列，拼接后获得2 287 bp的cDNA序列。TLR1基因系统进化树及同源性比对结果表明，TLR1基因极其保守，牦牛TLR1基因与黄牛、绵羊等哺乳动物遗传距离很近。预测TLR1基因含有1个2 184 bp的开放阅读框，编码727个氨基酸，其编码蛋白的分子质量为83.148 7 ku；预测TLR1蛋白在第500～600位氨基酸区域含有1个疏水区域，结合跨膜区预测认为该疏水区域可能是TLR1蛋白的一个跨膜区，TLR1蛋白二级结构主要以α-螺旋及自由卷曲为主。【结论】TLR1基因在高原牦牛与平原哺乳动物之间存在较高的同源性，这可能与TLR1蛋白重要的生理功能相关。 |
| 关键词: 麦洼牦牛 TLR1基因 克隆 生物信息学分析 |
| DOI： |
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| 基金项目:西南民族大学研究生创新性项目（CX2014SZ75） |
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| Cloning and bioinformatics analysis of Toll-like receptor 1 gene in Maiwa yak |
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CHEN Ya-bing,LAN Dao-liang,HUANG Cai,et al
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| Abstract: |
| 【Objective】This study cloned and analyzed the characteristics of TLR1 gene from Maiwa yak (Bos grunnien).【Method】RNA from yak spleen was extracted and cDNA was synthesized by reverse transcription reaction.TLR1 gene was divided into two sections for amplification.The sequence characteristics of TLR1 gene and basic physical and chemical characters of encoded protein were predicted and analyzed by related software.【Result】The length of the first fragment and the second fragment were 1 484 bp and 823 bp,respectively,which combined to a cDNA fragment of 2 287 bp.Phylogenetic tree and sequence alignment showed the TLR1 gene of Maiwa yak had a high homology with other species in nucleotide sequence and the gene was relatively conservative.The gene was predicted to have an ORF (2 184 bp) encoding 727 amino acids with a molecular mass of 83.148 7 ku.The protein was also predicted to contain a hydrophobic region at the area of No.500-600 amino acid,which may be a transmembrane domain of TLR1 protein.Moreover,the secondary structures of the protein were mainly composed of a-helix and random coli.【Conclusion】TLR1 gene in yak shared high homology with plain mammals,indicating that TLR1 gene may play an important role in mammals. |
| Key words: Maiwa yak TLR1 gene cloning bioinformatic analysis |
