| 摘要: |
| 【目的】以软枣猕猴桃优良品系“2008-07”嫩茎段为外植体，研究其组织培养繁育技术。【方法】采用均匀试验设计，通过组培快繁试验，对影响软枣猕猴桃优良品系“2008-07”嫩茎段腋芽萌发生长和生根的6-BA、IAA、IBA等生长调节物质的作用及其用量进行分析与筛选。【结果】适宜软枣猕猴桃嫩茎段腋芽萌发生长、生根的培养基为基本培养基＋0.12 mg/L 6-BA＋0.04 mg/L IAA，在此条件下植株再生率为98.7%。再生植株的移栽成活率达99.5%以上。【结论】建立了软枣猕猴桃优良品系“2008-07”嫩茎段组培快繁育苗体系，该体系适合于软枣猕猴桃种苗的工厂化生产。 |
| 关键词: 软枣猕猴桃 组织培养 繁殖 优良品系 均匀设计 |
| DOI： |
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| 基金项目:吉林省大学生创新创业训练计划项目(2013THSY06) |
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| Tissue culture and propagation technique for fine strain (2008-07) of Actinidia arguta (Sieb.et Zucc.) Planch.ex Miq. |
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GUO Zhi-xin,GU Di-zhou,YAN Zhong-xue,et al
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| Abstract: |
| 【Objective】Young stems of Actinidia arguta fine strain “2008-07” were used as explants to investigate the tissue culture and propagation technique.【Method】Effects of p6-BA,IAA,and IBA)on bud sprouting,shoots growth and shoots rooting of fine strain “2008-07” were tested through uniform design experiments and tissue culture and propagation technique.【Result】The basic medium+6-BA 0.12 mg/L+IAA 0.04 mg/L was suitable for axillary sprouting,shoots growth and shoots rooting of young stems of Actinidia arguta fine strain “2008-07”.The plantlet regeneration rate and survival rate of regenerated plants were 98.7% and 99.5%, respectively.【Conclusion】The established micropropagation system for Actinidia arguta fine strain “2008-07” was suitable for industrialized seedling of Actinidia arguta. |
| Key words: Actinidia arguta (Sieb.et Zucc.) Planch.ex Miq. tissue culture propagation fine strain uniform design |
