| 摘要: |
| 【目的】在花生野生种、栽培种及栽野杂交后代中，克隆抗病基因类似物(Resistance gene analog,RGA)相关基因片段，为抗病基因的进一步分离、鉴定及利用奠定基础。【方法】以14个花生栽野杂交种和2个抗病花生亲本为材料，应用PCR方法扩增来自基因组DNA的抗病基因类似物序列，并分析其同源性。【结果】扩增出15条来自基因组DNA的抗病基因类似物序列，序列长度在500 bp左右，由此推导出的15条氨基酸序列含有典型的NBS保守区的N端糖基化位点、蛋白激酶C磷酸化位点、酪蛋白激酶Ⅱ磷酸化位点、N-豆蔻酰化位点。15条花生RGA氨基酸序列间的同源性在73.2%～100.0%，与已发表的花生抗病基因类似物的相似性较高，达72.1%～100.0%；与已知的其他作物的抗病基因编码的氨基酸序列有34.1%～54.6%的同源性。【结论】分离到的15个花生抗病基因同源片段为抗病基因的部分序列。 |
| 关键词: 花生 抗病基因类似物 克隆 |
| DOI： |
| 分类号: |
| 基金项目:国家现代农业产业技术体系建设专项（CARS-14-华南区栽培岗位）；广西青年基金项目（桂科青0991016）；广西重点实验室建设项目（12-071-09） |
|
| Cloning and analysis of resistance gene analogs in filial generation of genus Arachis |
|
LI Zhong,JIANG Li-geng,TANG Rong-hua
|
| Abstract: |
| 【Objective】Resistance gene analogs (RGAs) of wild peanut variety,peanut cultivar and their filial generation were cloned to provide basis for further separation,identification and utilization of peanut resistance genes.【Method】Resistance gene analogs of filial generation of genus Arachis and their parents were isolated using homology-based method.The resistance gene analogs were amplified using primers based on the conserved regions from reported peanut resistance genes.【Result】15 resistance gene analogs with length of 500 bp were obtained.The RGAs contained typical NBS conserved motifs,such as N glycosylation site,Protein kinase C phosphorylation site,Casein kinase Ⅱ phosphorylation site and N myristoylation site.The sequences of these 15 RGAs shared 73.2%－100.0% identity.They also showed high sequence identity (72.1%－100.0%) with reported peanut resistance genes.Putative amino acid sequences deduced from these 15 RGAs shared 34.1%－54.6% identity with the sequences of resistance genes of other plants.【Conclusion】The obtained 15 RGAs from genus Arachis belonged to sequences of the resistance genes. |
| Key words: peanut resistance gene analog clone |
