| 摘要: |
| 【目的】研究从腐烂紫菜中分离的Stenotrophomonas sp.Z705菌株产琼胶酶的最佳发酵条件和培养基组成。【方法】采用单因素试验，分析装液量、摇床转速、发酵时间、初始pH、发酵温度、盐度等因素对Stenotrophomonas sp.Z705菌株发酵产琼胶酶活力的影响，从中筛选出该菌株的最佳发酵条件。在此基础上，采用单因素试验和L9(33)正交试验，分析不同氮源和碳源对Stenotrophomonas sp.Z705菌株发酵产琼胶酶活力的影响，从中筛选出该菌株最佳培养基的组成。【结果】Stenotrophomonas sp.Z705菌株发酵产琼胶酶的最佳条件为：装液量25 mL、摇床转速200 r/min、发酵时间23 h、初始pH 7.2、发酵温度28 ℃、盐度3.4%；最佳培养基组成为：牛肉膏5.0 g/L、酵母浸膏 1.50 g/L、琼胶0.30 g/L。【结论】在最佳组成培养基和发酵条件下，该菌株产琼胶酶活力稳定在87.1 U/mL左右，比优化前提高了60.4%。 |
| 关键词: 寡养单胞菌属 琼胶酶产生菌 正交试验 酶活力 |
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| 基金项目:福建省重大科技专项“动物营养免疫调控技术集成及功能性饲料开发”(2010NZ0002) |
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| Fermentation optimization of agarase producing bacteria Stenotrophomonas sp.Z705 |
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MIAO Fu-rong,DONG Zhi-yan,LIU Jing,et al
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| Abstract: |
| 【Objective】This study aimed to investigate the optimal culture medium and fermentation conditions for agarase producing bacteria Stenotrophomonas sp.Z705 extracted from rotting laver.【Method】Using single factor test,effects of liquid volume,shaking speed,fermentation time,initial pH,fermentation temperature and salinity on fermentation enzyme activity of Stenotrophomonas sp.Z705 strains were analyzed and the optimal fermentation conditions were obtained.Using single factor test and L9(33) orthogonal test,effects of different nitrogen sources and carbon sources on fermentation enzyme activity of Stenotrophomonas sp.Z705 strains were also analyzed to screen the optimal composition of culture medium.【Result】The best conditions were:liquid volume of 25 mL,shaking speed of 200 r/min,fermentation time of 23 h,initial pH of 7.2,fermentation temperature of 28 ℃,and salinity of 3.4%.The optimal culture medium composition was:beef extract 5.0 g/L,yeast extract 1.50 g/L and agar 0.30 g/L.【Conclusion】With the optimum culture medium and fermentation conditions,the fermentation enzymatic activity of agarase producing bacteria Stenotrophomonas sp.Z705 strains was 87.1 U/mL,60.4% higher than before optimization. |
| Key words: Stenotrophomonas sp. agarase-produced bacterium orthogonal test enzyme activity |
