| 摘要: |
| 【目的】建立黄瓜的高频再生体系，为黄瓜种质的遗传转化奠定基础。【方法】分别以华北型黄瓜种质26号、欧洲型黄瓜种质14 1为材料，研究不同激素组合、AgNO3质量浓度、苗龄、子叶切割方式及外植体接种方式对黄瓜子叶节不定芽诱导的影响。 【结果】华北型黄瓜种质26号再生的最适激素组合为4.0 mg/L 6-BA+0.3 mg/L IAA+1.0 mg/L AgNO3，再生率与再生系数分别可达85.00％和2.51；欧洲型黄瓜种质14-1再生的最适激素组合为3.0 mg/L 6-BA+2.0 mg/L ABA+1.0 mg/L AgNO3，再生率与再生系数分别可达86.70%和2.77；5 d苗龄的黄瓜子叶作为外植体时诱导不定芽的状态最佳；切除子叶端部2/3且基部附带有1 mm子叶柄、以叶背向下子叶柄微插入的方式进行接种再生效率最高。【结论】建立了黄瓜种质14-1和26号材料的高频再生体系，再生率均可达85％以上。 |
| 关键词: 黄瓜 再生体系 6-BA ABA IAA |
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| 基金项目:国家自然科学基金项目(31071813)；陕西省留学人员优先资助项目(A289021202)；西北农林科技大学高校基本科研业务费(QN2011088)；西北农林科技大学国际合作基金项目（A213021102） |
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| Establishment of a high-frequency regeneration system for cucumber |
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WAN Xu-hua,CHEN Shu-xia,SHEN Xiao-qing,et al
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| Abstract: |
| 【Objective】This study aimed to establish a high frequency and rapid regeneration system for cucumber.【Method】Using inbred lines of North China type 26 and Europe type 14 1,the effects of hormone combination,AgNO3 mass concentration,seedling age,cutting way and inoculation way on regeneration rate of cucumber cotyledonary node were studied.【Result】The optimum hormone combination for regeneration of North China type 26 was 4.0 mg/L 6-BA+0.3 mg/L IAA+1.0 mg/L AgNO3,with regeneration rate of 85.00％ and regeneration coefficient of 2.51.The best hormone combination for regeneration of Europe type 14-1 was 3.0 mg/L 6-BA+2.0 mg/L ABA+1.0 mg/L AgNO3,with regeneration rate of 86.70％ and regeneration coefficient of 2.77.The optimal seedling age for cotyledons regeneration was 5 d,and the best inoculation method was cutting two-thirds of front end with 1 mm cotyledonary petiole and inserting it to the medium with leaf back down.【Conclusion】A regeneration system with rate of >85% was established for North China type 26 and Europe type 14-1. |
| Key words: cucumber in vitro regeneration system 6-BA ABA IAA |
