| 摘要: |
| 【目的】明确白菜细胞质雄性不育类型、恢保关系及其多样性。【方法】采用遗传学方法和已报道的5个不育细胞质orf138、orf222、orf224、orf263和orf288的特异引物的分子鉴定方法，对8种大白菜雄性不育材料（CMS3411-7、CMS7311、00S54-m-1、00S54-m-2、12XC4、00S11-1、00S11-3和00S11-8）进行鉴定。【结果】恢保关系分析表明，Pol CMS的恢复系和保持系对于Ogu类型(12XC4)及Ogu和Nap综合类型(00S54-m-1、00S54-m-2、00S11-1、00S11-3和00S11-8)雄性不育（简称Ogu-Nap CMS）均表现为保持系。分子鉴定表明，8份白菜雄性不育材料中有2份的细胞质属于Pol型甘蓝型油菜不育类型，1份属于Ogu型萝卜不育类型，5份材料的细胞质为Ogu-Nap CMS不育胞质综合类型，没有芥菜不育细胞质。细胞质多样性分析结果表明，5份Ogu-Nap型甘蓝型不育胞质综合类型的细胞质雄性不育材料中，1份材料(00S54-m-1)在BnTR4位点存在变异。【结论】明确了白菜细胞质雄性不育类型、恢保关系及其细胞质多样性。 |
| 关键词: 大白菜 细胞质雄性不育 恢保关系 分子鉴定 |
| DOI： |
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| 基金项目:国家自然科学基金项目（30871717）；国家科技支撑计划项目（2012BAD02B01）；陕西省科技统筹创新工程计划项目 |
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| Restoring and maintaining relationship and molecular identification of cytoplasmic male sterility in Chinese cabbage |
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WANG Guo-fang,ZHANG Lu-gang,LI Dong-xia
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| Abstract: |
| 【Objective】The aim of this study was to clarify the sterile type,restoring and maintaining relationship,and diversity of cytoplasmic male sterility in Chinese cabbage.【Method】Genetic and molecular methods with special primers of the reported 5 sterile cytoplasms,orf138,orf222,orf224,orf263 and orf288,were used to identify the cytoplasmic male sterility of eight Chinese cabbages (CMS3411-7,CMS7311,00S54-m-1,00S54-m-2,12XC4,00S11-1,00S11-3,and 00S11-8).【Result】Both restorer and maintainer of Pol CMS acted as maintainer for Ogu CMS (12XC4) and comprehensive Ogu-Nap CMS (00S54-m-1,00S54-m-2,00S11-1,00S11-3,and 00S11-8).Molecular identification showed that two CMS materials belonged to Pol CMS type,one CMS material belonged to the Ogu CMS type and five CMS materials belonged to Ogu-Nap CMS type.There was no Brassica juncea CMS type.Analysis on cytoplasmic diversity showed that one out of the five Ogu-Nap CMS (00S54-m-1) had variation at BnTR4 site.【Conclusion】The sterile type,restoring and maintaining relationship and cytoplasmic diversity of eight CMS in Chinese cabbages were determined. |
| Key words: heading Chinese cabbage CMS restoring and maintaining relationship molecular identification |
