| 摘要: |
| 【目的】基于核糖体蛋白基因（rp）和运输蛋白基因（secY），明确了陕西、山西及山东3省枣疯植原体的亚组分类地位。【方法】采集陕西、山西及山东3省枣产区的枣疯病样品，提取样品总DNA；设计引物对rpF1、rpF2、rpR1和secF1、secF2，对枣疯植原体的rp基因和secY基因进行PCR扩增及核苷酸序列测定与系统发育分析。【结果】获得枣疯植原体的rp基因和secY基因，大小分别为1 196 和1 421 bp。序列比对结果表明，陕西、山西及山东3个省枣疯植原体的rp基因和secY基因序列均一致；系统发育分析表明，这3省的枣疯植原体与樱桃致死黄化植原体(CLY5)和桃树黄化植原体印度株系（PY-In）的亲缘关系最近。【结论】以rp和secY基因作为植原体16S rRNA基因分类系统的辅助分子证据，将陕西、山西、山东的枣疯植原体归属到16SrⅤ-B亚组，明确三省内枣疯植原体的亲缘关系。 |
| 关键词: 枣疯病 植原体 rp基因 secY基因 序列分析 |
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| 基金项目:林业公益性行业科研专项（201004003-4）；教育部长江学者和创新团队发展计划项目 |
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| Sequence analyses of rp and secY genes of phytoplasma of jujube witches broom |
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ZHANG Lei,HAN Xiang,SUI Dan-dan,et al
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| Abstract: |
| 【Objective】The subgroups of phytoplasmas of jujube witches broom (JWB) in Shaanxi,Shanxi and Shandong provinces were identified based on the rp and secY genes.【Method】Stem samples of jujube trees with witches broom were collected in Shaanxi,Shanxi and Shandong,and the total DNA of each sample was extracted by CTAB method.Primers of rpF1,rpF2,rpR1 and secF1,secF2 were designed to conduct the amplification of rp and secY genes,the sequences of nucleotides and the phylogenetic analysis.【Result】rp genes with 1 196 bp and secY genes with 1 421 bp of phytoplasmas were obtained.Sequences and phylogeny analyses indicated that the sequences of rp and secY genes of JWB phytoplasmas in Shaanxi,Shanxi and Shandong were identical.The JWB phytoplasmas in Shaanxi,Shanxi and Shandong were phylogenetically related to phytoplasmas of cherry lethal yellows (CLY5) and peach yellows (PY-In).【Conclusion】Based on rp and secY genes,JWB phytoplasmas in Shaanxi,Shanxi and Shandong were classified into subgroup 16SrⅤ-B. |
| Key words: jujube witches’ broom phytoplasma rp gene secY gene sequence analysis |
