| 摘要: |
| 【目的】对甘蓝型油菜Ogu CMS恢复基因（Rfo）设计的特异分子标记进行检测，为选育有别于国外的同类恢复材料提供依据。【方法】以甘蓝型油菜Ogu CMS 1575A的保持系及其恢复系1601、SR36、SR58、1628为材料，选取1个基于Ogu CMS 恢复基因设计的特异分子标记和5个与恢复基因连锁的萝卜标记，对甘蓝型油菜Ogu CMS 1575A 的4个恢复系进行恢复基因Rfo分子标记检测，并与国外同类恢复系RRH1、R113、R2000的检测结果进行初步比较。【结果】甘蓝型油菜Ogu CMS 1575A的4个恢复系1601、SR36、SR58、1628均存在 Ogu CMS 恢复基因。与Rfo连锁的萝卜基因区段的分子标记检测比较表明，恢复系1601、1628连锁的萝卜片段长度短于国外同类恢复系 R113；恢复系SR36、SR58与RRH1连锁的萝卜片段长度相近，但是SR58却缺少1601、1628、SR36共有的1个标记SG34。【结论】甘蓝型油菜Ogu CMS 1575A的恢复系1601、SR58、1628可能是有别于国外同类恢复系RRH1、R113、R2000的萝卜质恢复材料。 |
| 关键词: 甘蓝型油菜 Ogu CMS 恢复系 萝卜片段 分子标记 |
| DOI： |
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| 基金项目:陕西省科技统筹创新工程计划项目“主要粮油作物新品种选育”（2011KTZB02-01-03） |
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| Preliminary research on molecular markers of four Ogu CMS 1575A restorers in Brassica napus |
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ZHANG Bo,DONG Jun-gang,HU Yong-min,et al
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| Abstract: |
| 【Objective】This study detected the specific markers designed for Ogu CMS restoring gene (Rfo) to provide a reference for breeding restorer materials different from similar foreign restorer materials.【Method】Taking the maintainers and the four restorer lines 1601,SR36,SR58,and 1628 of Ogu CMS 1575A in Brassica napus as materials,one specific molecular marker designed for Ogu CMS restoring gene (Rfo) and five radish molecular markers linked to Rfo were picked to detect the molecular markers of four Ogu CMS 1575A restorers and compare them with similar foreign restorers (RRH1,R113,and R2000).【Result】The four restorer materials 1601,SR36,SR58,and 1628 all contained Ogu CMS restorer gene.Comparing with the radish molecular markers linked to Rfo,the lengths of radish fragments in 1601 and 1628 were shorter than that of similar foreign restorer line R113.SR36 and SR58 had similar length of radish fragment to RRH1,but SR58 lacked marker SG34 that 1601,1628 and SR36 had.【Conclusion】1601,SR58,and 1628 were different from similar foreign restorer lines RRH1,R113,and R2000. |
| Key words: Brassica napus Ogu CMS restorer radish-fragment molecular markers |
