| 摘要: |
| 【目的】研究谷胱甘肽（GSH）还原亚硝酸钠（NaNO2）生成S-亚硝基谷胱甘肽（GSNO）所释放NO对玉米幼苗抗旱性的影响。【方法】以玉米品种“郑单958”为供试材料，采用盆栽试验，分别用NNO2-+GSH合成的不同浓度GSNO（50,250,500,800,1 000 μmol/L）熏蒸处理三叶期的玉米幼苗，通过控制浇水进行干旱胁迫，以未经GSNO处理的玉米幼苗（0 μmol/L GSNO）为干旱对照，通过测定干旱胁迫期间玉米的生长、生理指标，分析GSNO对干旱胁迫条件下玉米植株生长、抗氧化酶活性、膜脂过氧化程度的影响。【结果】干旱胁迫7 d后，50～1 000 μmol/L GSNO处理的玉米幼苗较干旱对照生长更好，其中500 μmol/L GSNO处理玉米幼苗的株高、茎粗和叶面积分别较干旱对照提高32.26%，45.83%和53.13%。GSNO处理能够显著增强干旱胁迫玉米幼苗的超氧化物歧化酶（SOD）、谷胱甘肽还原酶（GR）、过氧化氢酶（CAT）和抗坏血酸氧化酶（APX）的活性，相应减少过氧化氢（H2O2）和超氧阴离子（O2-·）含量，降低膜脂的过氧化伤害。GSNO处理还能够维持干旱胁迫下玉米幼苗光合色素的相对稳定性，降低离体叶片的失水速率，其变化趋势表现为500 μmol/L<50 μmol/L<1 000 μmol/L。【结论】GSNO处理能明显改善玉米的抗旱性，其发挥作用的最佳浓度为500 μmol/L。 |
| 关键词: 玉米 谷胱甘肽 S-亚硝基谷胱甘肽 干旱胁迫 |
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| Alleviation of drought stress to maize by nitrite with the presence of Glutathione |
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XU Yi-xin,WANG Fei,ZHANG Cheng-shuang,et al
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| Abstract: |
| 【Objective】This article study the effects of NO released in the formation of S-nitroso-glutathione (GSNO) by Glutathione (GSH) and sodium nitrite (NaNO2) on resistance of maize seedlings (Zea mays L.) to drought stress.【Method】Pot planted Zhengdan 958 maize seedlings at three-leaf stage were treated with different concentrations of GSNO (50, 250,500,800,and 1 000 μmol/L).Drought stress was simulated by controlling water supply.Maize seedlings without GSNO were treated as control group.Growth and physiological indexes of maize seedlings were measured to analyze the influence of GSNO on plant growth,antioxidant enzyme activity and lipid peroxidation of maize.【Result】After 7 days of drought stress,the plants treated by 50-1 000 μmol/L GSNO grew better than the control.Compared to the control,500 μmol/L GSNO treatment improved height,stem diameter and leaf area of maize seedlings by 32.26%,45.83% and 53.13%,respectively.GSNO enhanced activities of antioxidant enzymes such as superoxide dismutase(SOD),glutathione reductases(GR),catalase(CAT) and ascorbate peroxidase(APX),while decreased both H2O2 and O2-· levels in maize leaves,and protected membrane lipids from over-oxidation.GSNO also maintained photosynthetic pigments and decreased water-losing of detached leaves with the trend of 500 μmol/L<50 μmol/L<1 000 μmol/L.【Conclusion】GSNO treatment significantly improved the drought resistance of maize,and the optimal concentration was 500 μmol/L. |
| Key words: maize GSH GSNO drought stress |
