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Cr3+对土壤酶活性的影响
张国庆1, 和文祥1, 吕家珑,等1
西北农林科技大学 资源环境学院
摘要:
【目的】研究Cr3+含量对土壤脲酶和脱氢酶活性的影响,以筛选出土壤Cr3+污染评价的酶学指标以及影响酶与Cr3+关系的主要因素。【方法】以采自全国的18种典型土样为材料,采用室内模拟方法,研究不同含量Cr3+对18种土壤脲酶和脱氢酶活性的影响。【结果】Cr3+总体上抑制了土壤脲酶和脱氢酶活性。拟合土壤脲酶和脱氢酶活性与Cr3+含量间的关系,结果显示二者呈显著或极显著负相关,且机理为完全抑制(包括竞争性抑制和非竞争性抑制)作用。计算获得Cr3+对脲酶和脱氢酶的临界污染浓度分别为16.38和4.32 mg/kg,远小于Cr3+污染国家二级标准。【结论】土壤脲酶和脱氢酶在一定程度上可表征土壤Cr3+污染的程度,土壤pH和有机质分别是影响Cr3+对土壤脲酶和脱氢酶毒害的主要因素。
关键词:  Cr3+  脲酶  脱氢酶  生态剂量  作用机理
DOI:
分类号:
基金项目:国家“863”高技术研究发展计划项目(2012AA101402);公益性行业(农业)科研专项(200903015);西北农林科技大学基本科研业务费科研创新重点项目(ZD2013012)
Effects of Cr3+ on soil enzyme activity
ZHANG Guo-qing,HE Wen-xiang,LÜ Jia-long,et al
Abstract:
【Objective】The effect of Cr3+ on activities of soil urease and dehydrogenase was studied to screen the enzymology indicators for soil Cr3+ pollution and the main factors influencing the relationship between enzymes and Cr3+.【Method】Using laboratory simulation method,we studied the effect of Cr3+ on activities of soil urease and dehydrogenase in 18 typical soil samples collected nationwide.【Result】Cr3+ generally inhibited the activities of urease and dehydrogenase with significant or extremely significant relations.The reaction was complete inhibition (including competitive inhibition and noncompetitive inhibition).The critical pollution concentrations of urease and dehydrogenase were 16.38 and 4.32 mg/kg respectively,which were lower than the second level of national standards.【Conclusion】To a certain extent,activities of soil urease and dehydrogenase can be used to characterize soil Cr3+ pollution.The soil pH and organic matter were the domain factors deciding the effects of Cr3+ on activities of soil urease and dehydrogenase,respectively.
Key words:  Cr3+  urease  dehydrogenase  ecological dose  interaction mechanism