| 摘要: |
| 【目的】研究酸枣果肉多糖在抗衰老、增强免疫及心血管功能方面的保健作用。【方法】制备酸枣果肉多糖备用，以小鼠为试验动物，采用皮下注射D 半乳糖的方法构建衰老模型小鼠，给衰老模型小鼠连续灌胃低剂量（250 mg/kg）和高剂量（500 mg/kg）酸枣果肉多糖30 d，测定血清中SOD、CAT活性和MDA含量，试验同时设空白对照组（给健康小鼠灌胃蒸馏水）、模型对照组（给衰老模型小鼠灌胃蒸馏水）和阳性对照组（给衰老模型小鼠灌胃维生素E 30 mg/kg）。给健康小鼠连续灌胃60 d低、高剂量酸枣果肉多糖，末次灌胃24 h后，一部分取样测脾脏指数、胸腺指数和碳粒廓清指数；一部分小鼠尾静脉注射垂体后叶素诱发心肌缺血，测定注射前后小鼠的心率。【结果】酸枣果肉多糖可抑制衰老模型小鼠血中SOD和CAT活性下降和MDA浓度升高，其中酸枣果肉多糖高剂量组小鼠血清中的SOD、CAT活性和MDA浓度分别为空白对照组的97.3%，99.5%和101.5%。与空白对照组相比，酸枣果肉多糖能增加小鼠免疫器官质量和碳粒廓清指数，其中酸枣多糖高剂量组的碳粒廓清指数是空白对照组的3.363倍；酸枣果肉多糖对垂体后叶素所致心肌缺血小鼠的心率增高有一定拮抗作用。【结论】酸枣果肉多糖对所试动物具有抗衰老、增强免疫功能及改善心血管的保健作用。 |
| 关键词: 酸枣果肉 多糖 抗衰老 免疫功能 心率 |
| DOI： |
| 分类号: |
| 基金项目:河北省科技支撑计划项目(No.13232809) |
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| Preliminary study on healthcare effects of wild jujube pulp polysaccharides |
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LANG Jie,CUI Na,ZHANG Li-bin
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| Abstract: |
| 【Objective】The healthcare effects of wild jujube pulp polysaccharides on anti-aging,immune and cardiovascular function were studied.【Method】Polysaccharides were extracted from the jujube pulp and reserved.Aging model mice were constructed with subcutaneous injection of D-galactose.Anti-aging experiment was carried out with low-dose (250 mg/kg) and high dose (500 mg/kg) feed of jujube pulp polysaccharides to aging model mice and normal mice for 30 days.SOD,CAT activities and MDA content in mice blood serum were measured.Three control groups,blank control group,model control group and positive control group were set up as well.After low and high dose jujube pulp polysaccharides were feed to the normal mice for 60 days,the weight of immune organs and carbon clearance index were measured in part of the mice,while the heart rate was measured in rest of the mice before and after injecting pituitrin for inducing myocardial ischemia.【Result】Jujube pulp polysaccharides inhibited SOD and CAT activity decreasing and MDA content increasing in the blood of galactose model mice (SOD,CAT activities and MDA content in jujube pulp polysaccharides high-dose group were 97.3%,99.5% and 101.5% of that in the blank control group,respectively).Immune organ weight and carbon clearance index were increased in mice fed with the polysaccharides (carbon clearance index in high-dose group was 3.363 times of that in blank control group).Jujube pulp polysaccharides had certain antagonistic effects on the increase of heart rate in myocardial ischemia mice induced by pituitary.【Conclusion】The healthcare effects of wild jujube pulp polysaccharides on anti-aging,immune and cardiovascular function were confirmed in the animal test. |
| Key words: wild jujube pulp polysaccharides anti-aging immune function heart rate |
