| 摘要: |
| 【目的】研究1，8-桉叶油素亚慢性中毒对小鼠体质量、血常规及血液生化指标的影响。【方法】将160只体质量15～17 g的小鼠随机分为4组，每组40只，分别灌胃剂量为192.45，64.15，21.38 mg/kg的1,8-桉叶油素和体积分数0.5%吐温 80水溶液（对照组），每组按10 g体质量0.2 mL给药，1次/d，连续灌胃染毒90 d，分别于给药第30，60，90天和停药后30 d测定小鼠体质量、血常规和血液生化指标。【结果】 与对照组相比，64.15和21.38 mg/kg 1,8 桉叶油素在试验期间对小鼠的体质量、血常规及血液生化指标的影响均无统计学差异(P＞0.05)；192.45 mg/kg 1,8 桉叶油素在灌胃60和90 d时，对小鼠的血常规和血液生化指标有不同程度的影响，多数指标与对照组差异显著(P＜0.05)；停药后30 d，1,8 桉叶油素各剂量组的血常规和血液生化指标与对照组差异不显著 (P＞0.05)。【结论】 1,8-桉叶油素对小鼠有亚慢性毒性作用，其有害作用剂量(NOAEL)为64.15 mg/kg，损害作用最低剂量(LOAEL)为192.45 mg/kg；1,8-桉叶油素对小鼠血液生化指标的影响在短期内是可逆的。 |
| 关键词: 1,8-桉叶油素 亚慢性毒性 损害作用剂量 有害作用剂量 |
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| 基金项目:四川省青年科技创新研究团队培育计划项目（2011JTD0035）；四川省教育厅成果转化培育项目（11ZZ022） |
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| Effects of sub-chronic intoxication of 1,8-cineole on blood biochemical indexes of mice |
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DU Yong-hua,WANG Chuan,ZHOU Li-jun,et al
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| Abstract: |
| 【Objective】Effects of sub-chronic intoxication of 1,8-cineole on body weights, routine blood indexes and biochemical indexes of mice were investigated.【Method】160 mice with body weights of 15-17 g were randomly divided into 4 groups (40 mice/group).Mice were subjected to 1,8-cineole with doses of 192.45, 64.15 and 21.38 mg/kg body weight (test groups) and the water solution of tween-80 with a volume fraction of 0.5% (control group),respectively.Each mouse was administered orally at the dose of 0.2 mL per 10 g body weight once a day consecutively for 90 d.The body weight,routine blood indexes and serum biochemical indexes of mice were determined at the end of the 30th d,60th d,90th d and the 30th h d after stopping the administration of 1,8-cineole.【Result】The effects of 1,8-cineole on the body weight,routine blood indexes and serum biochemical indexes of mice with the doses of 64.15 and 21.38 mg/kg body weight had no statistically significant difference compared with the control group (P＞0.05).1,8-cineole with the dose of 192.45 mg/kg body weight exhibited different influences on routine blood indexes and serum biochemical indexes of mice after the oral administration of 1,8-cineole for 60 d and 90 d,and statistically significant differences in many blood biochemical indexes were observed (P＜0.05).However,the differences in routine blood indexes and serum biochemical indexes were not statistically significant between the test groups and the control group at the 30th d after stopping the administration of 1,8-cineole(P＞0.05).【Conclusion】1,8-cineole had sub-chronic oral toxicity to mice. The no observed adverse effect level (NOAEL) of 1,8-cineole was 64.15 mg/kg body weight and the lowest observed adverse effect level (LOAEL) of 1,8-cineole was 192.45 mg/kg body weight.Effects of1,8-cineole on blood biochemical indexes of mice in short term were reversible. |
| Key words: 1,8-cineole sub-chronic toxicity lowest observed adverse effect level no observable adverse effect level |
