| 摘要: |
| 【目的】探究西葫芦未受精子房离体培养的主要影响因素，以提高其胚状体诱导率和生根品质，增加再生植株数量，丰富西葫芦育种材料。【方法】以西葫芦“春玉一号”未受精子房为材料，分析MS、B5、N6和White 4种基本培养基的诱胚效果；在MS培养基中添加不同质量浓度的6-BA（0，0.5，1.0，2.0 mg/L）和NAA（0，0.2，0.5，1.0 mg/L），筛选2种激素的最佳组合；之后添加不同质量浓度的2,4 D（0，1.0，2.0，3.0，4.0，5.0 mg/L），筛选2,4 D的适宜质量浓度；在MS培养基中添加不同质量浓度的NAA（0，0.05，0.1，0.2，0.5，1.0 mg/L），分析其对胚状体芽生根效果的影响。【结果】西葫芦未受精子房以MS为基本培养基时胚状体诱导率最高，为16.67%；在MS培养基中添加1.0 mg/L 6-BA+0.5 mg/L NAA时的诱导效果最好，诱导率为19.76%；在MS+30 g/L蔗糖+8 g/L琼脂+1.0 mg/L 6-BA+0.5 mg/L NAA培养基中添加1.0 mg/L 2,4-D可以加快胚珠膨大的速度，增加胚珠转绿的数量，但对出胚的提高效果不显著。MS+30 g/L蔗糖+8 g/L琼脂+0.1 mg/L NAA有利于提高胚状体芽的生根品质，且根系生长健壮。【结论】MS+30 g/L蔗糖+8 g/L琼脂+1.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L 2,4-D是西葫芦未受精子房离体培养中胚状体诱导的最佳培养基，MS+30 g/L蔗糖+8 g/L琼脂+0.1 mg/L NAA是诱导其胚状体芽生根的较适宜培养基。 |
| 关键词: 西葫芦 未受精子房 培养基 激素组合 胚状体诱导 |
| DOI： |
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| 基金项目:国家大宗蔬菜产业技术体系项目（CARS-25）；公益性行业（农业）科研专项（201303112） |
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| Factors affecting in vitro culture of unpollinated ovary of summer squash(Cucurbita pepo L.) |
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CHENG Hui, CHENG Yongan, ZHANG Enhui
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NorthwestA&FUniversity
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| Abstract: |
| 【Objective】Major factors affecting isolated culture of unfertilized ovary of summer squash (Cucurbita pepo L.) were investigated to improve the induction rate of embryoid and rooting quality,increase the number of regeneration plants,and enrich its breeding materials.【Method】Taking the unpollinated ovaries of ‘Chun Yu No.1’ as explants,we analyzed induction effects of MS,B5,N6 and White on embryoids.MS medium was supplemented with combinations of 6-BA (0,0.5,1.0 and 2.0 mg/L) and NAA (0,0.2,0.5 and 1.0 mg/L).Then 2,4 D（0,1.0,2.0,3.0,4.0 and 5.0 mg/L）was added to identify the best combination and optimum 2,4-D concentration.At last,effects of adding NAA (0,0.05,0.1,0.2,0.5 and 1.0 mg/L) to MS medium on root growth of embryoid bud were analyzed.【Result】The highest embryoid induction rate of unpollinated ovaries on basic medium was 16.67% on MS.The best embryoid induction rate was 19.76% on medium of MS+30 g/L Suc+8 g/L Agar+1.0 mg/L 6-BA+ 0.5 mg/L NAA.Adding 1.0 mg/L 2,4-D to the medium accelerated the inflation without improving the growth of embryo.MS+30 g/L Suc+8 g/L Agar+0.1 mg/L NAA enhanced the root growth of embryoid bud.【Conclusion】MS+30 g/L Suc+8 g/L Agar+1.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L 2,4-D was the best embryoid induction medium for in vitro culture of unfertilized ovary of summer squash.MS+30 g/L Suc+8 g/L Agar+0.1 mg/L NAA was suitable for root growth of embryoid bud. |
| Key words: summer squash (Cucurbita pepo L.) unpollinated ovary medium hormone combination embryoid induction |
