| 摘要: |
| 【目的】明确从特殊生境中获得的生防放线菌的耐药性及其生物学习性，为生防菌的筛选提供新思路。【方法】通过比较6种供试生防放线菌各种发酵液的抑菌活性及发酵产物的干物质量，初步确定其最佳发酵和营养条件；用对峙法测定供试生防菌的耐药性及抑菌谱；用平板稀释法测定供试生防菌在盆栽试验中的根际定殖能力。【结果】发酵和营养条件试验表明，高氏1号为菌株JSJF7的最佳培养液，菌株JSGF10、GXDM6、JSGF4、JSCK14和JSJF5的最佳培养液均为SNB；供试生防菌对50%多菌灵、40%五氯硝基苯和25%氰烯菌酯的耐药性较强；供试生防菌对选用的多种靶标病原菌均表现出一定的抑菌活性，抑菌谱较广；供试的6株生防菌均能在茄子根际定殖，但以菌株GXDM6的定殖能力最强。【结论】从特殊生境中获取耐药生防菌使菌药混用成为可能，同时也为特种有益菌的筛选提供了新思路。 |
| 关键词: 特殊生境 拮抗放线菌 耐药性 生物学习性 发酵条件 |
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| 基金项目:教育部创新团队发展计划项目（200558）；西北农林科技大学创新团队项目 |
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| Biological features of tolerant biocontrol actinomycetes from the special environment |
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| Abstract: |
| 【Objective】This study explicated the pesticide tolerance and biological features of biocontrol actinomycetes which were isolated from special environments,aiming to develop a new method for screening biological control strains.【Method】The conditions of fermentation and nutrition were preliminarily determined by analyzing and comparing the suppressive activities of the fermentation broth and the dry weight of fermentation products of candidate biocontrol actinomycetes.The pesticide tolerance and inhibition spectrums of candidate strains with bio-control ability were tested on agar plate and the colonization ability in pot experiment was tested using dilution plate method.【Result】The test demonstrated that Gause’s NO.1 medium was optimal for the strain JSJF7 and SNB was the optimal culture media for strains JSGF10,GXDM6,JSGF4,JSCK14 and JSJF5.The candidate bio-control strains showed strong tolerance to 50% benomyl,40% PCNB and 25% JS399-19.The candidate strains showed antagonistic activity to the tested target pathogens,which suggested that they had wider inhibition spectrum.They could colonize in the rhizosphere of eggplant and the strain GXDM6 showed stronger colonization ability.【Conclusion】The biological control strains with pesticide tolerance from special environments were obtained and integrated use of bio-control strains culture and pesticides could be achieved.The research also provided a new idea for screening the strains with specific functions. |
| Key words: special environments antagonistic actinomycetes pesticide tolerance biological habits fermentation conditions |
