| 摘要: |
| 【目的】探讨体细胞核移植（即克隆）操作对克隆猪心脏发育的影响。【方法】分别对3头正常繁殖猪和3头成年克隆猪的心脏进行解剖学观察和组织切片分析。提取心脏蛋白样品，利用基于双向电泳-质谱体系的比较蛋白质组学技术对心脏蛋白进行分析。【结果】解剖学观察和组织切片分析证实，克隆猪心脏发育异常；与正常繁殖猪相比，克隆猪心脏蛋白共有6种差异表达蛋白，分别为组织蛋白酶-D、骨骼肌型α-肌动蛋白、心肌肌球蛋白重链、热休克蛋白-27、Telethonin和骨骼肌型α-肌动蛋白，其中前5种蛋白表达上调，后1种蛋白表达下调。【结论】一些重要的心脏蛋白表达异常可能导致了克隆猪的心脏发育缺陷，比较蛋白质组学手段可以作为探索体细胞核移植机制的重要方法。 |
| 关键词: 猪 克隆 体细胞核移植 心脏 比较蛋白质组学 |
| DOI： |
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| 基金项目:国家转基因生物新品种培育科技重大专项（2008ZX08006-005，2009ZX08006-014B）；上海市科委农业科技成果转化项目(103919N1800)；上海市农业科学院青年科技项目（2011(07)） |
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| Comparative proteomics of differentially expressed proteins from hearts of cloned and normal reproductive adult pigs |
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ZHANG Shushan, DAI Jianjun, WU Caifeng
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Shanghai Academy of Agricultural Sciences
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| Abstract: |
| 【Objective】The aim of this study was to explore the influence of somatic cell nuclear transfer (namely clone) operation on cardiac development.【Method】The anatomical structure and histological section of hearts from three cloned adult pigs and three normal reproductive pigs were investigated.The proteins from those pigs were analyzed using comparative proteomics containing two dimensional gel electrophoresis (2-DE) and mass spectrometry (MS).【Result】There were 6 differential expressed proteins (5 up-regulated and 1 down-regulated) between the cloned and normal reproductive pigs.Abnormal expression of proteins including Cathepsin D protein (CTSD),Actin,alpha skeletal muscle,Myosin heavy chain,cardiac (MHC),Heat shock protein-27 and Telethonin were found in cloned pigs.【Conclusion】Abnormal expression of some important proteins could result in abnormal development of hearts in cloned pigs,and comparative proteomics might be an important method to explore the mechanism of somatic cell nuclear transfer (SCNT). |
| Key words: pig clone somatic cell nuclear transfer heart comparative proteomics |
