| 摘要: |
| 【目的】研究甘肃主要肉用绵羊品种(波德代羊、无角陶赛特、蒙古羊、滩羊、小尾寒羊)DNA分子水平上的遗传多样性。【方法】利用世界粮农组织和国际家畜联合会推荐的11对微卫星引物（CSRD0298、RM0509、URB0038、MCM0130、ILSTS004、MCMA008、MNS0094、CSSM0004、OarAE101、BM1329和OarFCB11），对5个肉用绵羊品种分子水平上的遗传多样性及其与生产性能的关联性进行研究。【结果】11个微卫星位点在5个肉用绵羊品种间均呈现高度多态性，构建的系统进化树NJ聚类图将国内的3个肉用绵羊品种（蒙古羊、滩羊、小尾寒羊）与引进的2个肉用绵羊品种（波德代羊、无角陶赛特）分为2支；系统进化树UPGMA聚类图和主成分的二维散点图进一步证实了NJ聚类结果的可靠性。生产性能关联性分析结果显示，绵羊群体的平均产羔数与微卫星位点OarAE101存在关联性，与位点BM1329没有关联性；绵羊初生质量与微卫星位点ILSTS004和CSSM0004存在关联性。【结论】5个肉用绵羊品种具有丰富的遗传多样性，有很大的选育潜力。 |
| 关键词: 肉用绵羊 微卫星标记 生产性能 关联性 |
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| 基金项目:国家现代肉羊产业技术体系专项(11004986)；国家“863”高技术研究发展计划项目(SQ2010AA1000688008) |
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| Microsatellite markers genetic diversity and growth traits correlation of five meat sheep breeds in Gansu |
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| Abstract: |
| 【Objective】This study aimed to study the genetic diversity of main meet sheep (Borderdale,Poll Dorset,Mongolian Sheep,Tan Sheep,and Small Tail Han sheep) in Gansu.【Mehtod】11 microsatellite DNA markers (CSRD0298,RM0509,URB0038,MCM0130,ILSTS004,MCMA008,MNS0094,CSSM0004,OarAE101,BM1329 and OarFCB11) recommended by FAO and the international federation of livestock were used to detect the genetic variation and the association of growth performance in the five sheep breeds.【Result】Eleven of all the microsatellite loci were highly polymorphic.Neighbour-joining (NJ) dendrogram clustered Chinese indigenous meat sheep breeds and introduced breeds into two groups.The classification was further supported by both UPGMA dendrogram and scatter plots of estimates from principal component analyses.Association of growth performance revealed that the average litter size was related to OarAE101 microsatellite loci rather than BM1329.Both microsatellite loci ILSTS004 and CSSM0004 were related to lamb birth weight.【Conclusion】These results would benefit resource protection and development of these meat sheep breeds. |
| Key words: meat sheep microsatellite DNA growth performance relevance |
