| 摘要: |
| 【目的】探讨健脾益气汤对免疫抑制小鼠免疫功能的影响。【方法】给小鼠皮下注射环磷酰胺（CTX）30 mg/(kg·d)，制备免疫抑制模型；阴性对照组小鼠灌胃生理盐水，阳性对照组灌胃0.1 g/(kg·d)左旋咪唑，健脾益气汤低剂量给药组(16 g/(kg·d))和高剂量给药组(44 g/(kg·d))分别灌胃健脾益气汤，研究健脾益气汤对小鼠体质量、脾脏指数以及血清中IgG、IL-2、TNF-α质量浓度和T淋巴细胞亚群中CD4+、CD8+数量的影响。【结果】健脾益气汤低剂量给药组和高剂量给药组均能拮抗由环磷酰胺所导致的体质量下降，健脾益气汤高剂量组显著降低了免疫抑制小鼠的脾脏指数。健脾益气汤可以明显提高免疫抑制小鼠血清中的IgG、IL-2和TNF-α质量浓度，增加免疫抑制小鼠外周血中T淋巴细胞CD4+数量，降低CD8+数量，使CD4+8+提高。【结论】健脾益气汤能增强免疫抑制小鼠的免疫功能。 |
| 关键词: 健脾益气汤 小鼠 免疫功能 T细胞亚群 流式细胞术 |
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| 基金项目:河北省高等学校科学技术研究项目（Z2011117）；张家口市科技攻关计划项目（12110037C-2） |
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| Immunoregulatory effect of Jianpi Yiqi decoction on immunosuppressive mice |
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| Abstract: |
| 【Objective】This study aimed to explore the immunoregulatory effect of Jianpi Yiqi (JPYQ) decoction on immunosuppressive mice.【Method】The immunosuppressive mouse model was established by hypodermic injection of cyclophosphamide with concentration of 30 mg/(kg·d).To investigate the effect of JPYQ decoction on weight,spleen index,mass concentrations of IgG,IL-2 and TNF-α in the blood serum,and CD4+ and CD8+ in T lymphocyte subgroup of mice,mice with normal saline and levamisole were selected as negative and positive control groups respectively and mice fed with JPYQ decoction of 16 g/(kg·d) and 44 g/(kg·d) were considered as low dose group and high dose group,respectively.【Result】High dose of JPYQ decoction could raise spleen index of immunosuppressive mice significantly.Both low and high dose groups could resist the weight decrease caused by cyclophosphamide.It could also raise the concentrations of IgG,IL-2,and TNF-α,and the quantity of CD4+ but decrease quantity of CD8+ which resulted in elevated CD4+ /CD8+.【Conclusion】JPYQ decoction had positive effects on immune function of immunosuppressive mice. |
| Key words: Jianpi Yiqi decoction mouse immune function T cells subgroup flow cytometry |
