| 摘要: |
| 【目的】研究岚皋魔芋软腐病病原细菌生物多样性。【方法】采用组织分离法分离纯化魔芋软腐病球茎及叶柄中的致病菌，通过魔芋球茎、胡萝卜及马铃薯块的侵染试验初步确定其致病性，再利用魔芋球茎侵染致病及盆栽致病性试验确认其致病性；经菌落与细胞形态、生理生化特性及16S rRNA序列测定确定病原菌的类型。【结果】从魔芋软腐病球茎及叶柄中共分离获得16株细菌，侵染试验表明，其中CDS1-B1、CDS1-B2、CDS2-B1、CDS2-B2、CZS-B4和CZS-B6共6株细菌可使魔芋球茎表现软腐症状，并导致盆栽魔芋发病；6株细菌的菌落与细胞形态均存在差异；在魔芋、胡萝卜及马铃薯块上的侵染能力不同；16S rRNA序列分析表明，菌株CDS1-B1、CDS1-B2、CDS2-B1及CDS2-B2均与菊欧氏杆菌(Erwinia chrysanthemi)亲缘关系最近，菌株CZS B4与菊欧文氏菌(Dickeya dadantii)亲缘关系最近，相似度为98.8%，菌株CZ-B6与菊果胶杆菌(Pectobacterium chrysanthemi)的相似度达99.9%。【结论】导致岚皋县魔芋软腐病的致病细菌存在生物多样性，其致病性也存在差异，其中新发现的魔芋软腐病原菌菊果胶杆菌(Pectobacterium chrysanthemi)致病性最强，菊欧氏杆菌(Erwinia chrysanthemi)和菊欧文氏菌(Dickeya dadantii)致病性较弱。 |
| 关键词: 魔芋软腐病 菊果胶杆菌 菊欧文氏菌 菊欧氏杆菌 |
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| 基金项目:国家林业局重点科技推广项目（魔芋产业化配套技术2010-38） |
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| Pathogenic bacteria biodiversity of Amorphophallus infected with soft rot disease in Langao,Shaanxi |
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| Abstract: |
| 【Objective】We studied the pathogenic bacteria biodiversity of Amorphophallus plants infected with soft rot disease in Langao,Shaanxi.【Method】Soft rot bacteria in corm of Amorphophallus was isolated by conventional tissue method,the pathogenic ability was identified by infection test on Amorphophallus,carrot,potato tissues,Amorphophallus corms and healthy Amorphophallus planted in the pots.Pathogens were identified by colony and cell morphologies,16S rRNA sequence and biological characters.【Result】Sixteen strains from Amorphophallus corms were obtained,six of which caused soft rot of Amorphophallus corms in infection test,and potted Amorphophallusi plants could be infected with soft rot disease through the six bacteria.There were differences in colony and cell morphologies of the six bacteria,and the infection abilities on the Amorphophallus,carrot and potato tissues were different.16S rRNA sequence analysis showed that CDS1-B1,CDS1-B2,CDS2-B1 and CDS2-B2 were related to Erwinia chrysanthemi/i>.Strain CZS-B4 was close to Dickeya dadantii (98.8%),and strain CZS-B6 was close to Pectobacterium chrysanthemi (99.9%).【Conclusion】Pathogens causing soft rot to i<>Amorphophallus plants in Laogao County existed biological diversity and had differences in infection ability.Newly discovered Pectobacterium chrysanthemi had high capacity to cause soft rot disease,while Erwinia chrysanthemi and Dickeya dadantii had relatively weak pathogenicity. |
| Key words: Amorphophallus soft rot disease Pectobacterium chrysanthemi Dickeya dadantii Erwinia chrysanthemi |
