| 摘要: |
| 【目的】建立葡萄风信子体细胞胚再生体系，通过外部形态判断愈伤组织的类型，并探索胚性愈伤组织对头孢霉素和潮霉素的敏感性。【方法】以多年生葡萄风信子‘蓝穗’的盛花期叶片为试验材料，通过正交试验筛选胚性愈伤组织诱导时适宜的植物生长调节剂质量浓度及配比；石蜡切片法观察不同愈伤组织内部的细胞结构,诱导具有胚性的愈伤组织形成体细胞胚，进而获得再生植株；在培养基中加入头孢霉素或潮霉素，观察2种抗生素对胚性愈伤组织生长和体细胞胚发生的影响。【结果】葡萄风信子胚性愈伤组织诱导的适宜培养基为MS+0.5 mg/L 2,4-D+0.1 mg/L TDZ，在该培养基上诱导率高达100%。石蜡切片观察发现，外观质地紧密、节状或块状的愈伤组织为胚性愈伤组织。胚性愈伤组织在不含任何激素的MS培养基上可诱导形成体细胞胚，在光照条件下体细胞胚伸长并生根，最后形成成熟小鳞茎。头孢霉素质量浓度超过500 mg/L时，葡萄风信子胚性愈伤组织的生长和体细胞胚的发生受到强烈抑制;引起胚性愈伤组织褐变的潮霉素临界质量浓度为90 mg/L。【结论】立了葡萄风信子体细胞胚再生体系，90 mg/L潮霉素可作为区分转化与非转化葡萄风信子胚性愈伤组织的有效选择压。 |
| 关键词: 葡萄风信子 胚性愈伤组织 体细胞胚发生 抗生素敏感性 |
| DOI： |
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| 基金项目:西安园林观赏花卉示范园建设子项目(NC10031(2)) |
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| Plant regeneration from somatic embryogenesis in Muscari armeniacum Blue Spike and antibiotics sensitivity test |
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YANG Meifeng, WANG Fei, ZHANG Wen,et al
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Northwest A&F University
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| Abstract: |
| 【Objective】The research was to establish the regeneration system of Muscari armeniacum from somatic embryogenesis,identify the types of callus by appearances,and study the sensibilities of embryogenic callus to cefotaxime and hygromycin.【Method】Leaves of M.armeniacum‘Blue spike’ were used as experimental material,the optimal plant growth regulator concentration for embryogenic callus induction was screened with orthogonal design test.The inner cellular structures of various calluses were observed by paraffin method.Somatic embryos were induced from embryogenic callus,and plantlets derived from somatic embryos were achieved.Embryogenic callus and somatic embryos were cultured in medium with cefotaxime or hygromycin,and the effect of cefotaxime or hygromycin on them was observed.【Result】The best medium to induce embryogenic callus was MS+0.5 mg/L 2,4-D+0.1 mg/L TDZ,and the induction frequency was 100%.It was verified by paraffin method that callus with a close texture and nodular appearance was embryogenic callus.Somatic embryos were induced from embryogenic callus and converted to plantlets,rooted and mature bulbs formed on PGR-Free MS medium.Growth of embryogenic callus and somatic embryogenesis were strongly inhibited with more than 500 mg/L cefotaxime in the medium.The critical mass concentration to hygromycin was 90 mg/L.【Conclusion】Regeneration system in M.armeniacumfrom somatic embryognesis was obtained,and genetically modified embryogenic callus could be screened with 90 mg/L hygromycin. |
| Key words: Muscari armeniacum embryogenic callus somatic embryogenesis sensitivity to antibiotics |
