| 摘要: |
| 【目的】 利用基因枪将WZY2基因RNA干扰表达载体pAHC-WZY2-Ri导入“郑引1号”小麦，获得WZY2基因表达缺失型小麦，为深入分析WZY2基因功能奠定基础。【方法】以植物表达载体pAHC25为基础，构建含有反向重复序列的RNA干扰表达载体pAHC-WZY2-Ri，利用基因枪将其转入“郑引1号”小麦幼胚愈伤组织中，经过筛选、PCR检测得到阳性植株。【结果】从转化的1 500个愈伤组织中获得27株再生植株。利用PCR对再生植株进行检测，获得阳性植株3株，转化率为0.2%。【结论】构建了WZY2基因的RNA干扰表达载体，成功地将WZY2基因RNA干扰表达载体导入“郑引1号”小麦，获得阳性植株。 |
| 关键词: 小麦 WZY2基因 RNA干扰 遗传转化 |
| DOI： |
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| 基金项目:国家自然科学基金项目(31071349) |
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| Construction of RNAi expression vector of WZY2 gene from wheat and its genetic transformation |
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| Abstract: |
| 【Objective】The study aimed to obtain the transgenic wheat with the gene of WZY2 silenced by transforming WZY2into the RNAi expression vector,and provide information for further investigation of WZY2.【Method】Using pAHC25 as a basic vector,the RNAi expression vector of WZY2named pAHC-WZY2-Ri was constructed and introduced into wheat variety “Zhengyin No.1” by biolistic particle.Then the transgenic plants were identified by PCR.【Result】The 1 500 embryos of “Zhengyin No.1” were bombarded by biolistic particle,and 27 regenerated plants were obtained.Three of them were identified positive by PCR and the transformational frequency was 0.2%.【Conclusion】In this study,the RNAi expression vector of WZY2 was constructed,and was successfully transformed into “Zhengyin No.1”. |
| Key words: wheat WZY2gene RNA interference genetic transformation |
