| 摘要: |
| 【目的】研究VA对草鱼幼鱼生长、体成分和转氨酶活性的影响，以确定草鱼饲料中VA适宜的添加量。【方法】采用酪蛋白和脱脂豆粕为蛋白源、白糊精为糖源、玉米胚芽油和大豆油为脂肪源的半纯化饲料作为基础饲料，配制VA水平为0（对照组），810，1 620，2 520，3 224，3 980，7 950，16 386 IU/kg的8组试验饲料，饲养初始体质量为（10.79±0.52） g的草鱼12周，每组3个重复，每重复40尾，试验结束后测算体质量增长率、特定生长率、饲料系数、鱼体营养成分以及血清中碱性磷酸酶（ALP）、谷丙转氨酶（GPT）和谷草转氨酶（GOT）活性等指标。【结果】对照组的草鱼幼鱼，在试验后期有8.33%出现眼球突出、尾鳍充血的症状，添加VA试验组的草鱼未出现类似症状；饲料中VA含量在0～1 620 IU/kg时，体质量增长率随饲料中VA增加而显著增加（P＜0.05），饲料中VA含量>7 950 IU/kg时，体质量增长率显著降低（P＜0.05）；特定生长率的变化趋势与体质量增长率类似，而饲料系数变化趋势与体质量增长率相反；VA对草鱼成活率和全鱼水分、蛋白质、脂肪、灰分含量无显著性影响（P＞0.05）；饲料中缺乏VA会显著降低血清中ALP的活性，同时显著提高GPT和GOT的活性（P＜0.05）。【结论】在本试验条件下，饲料中缺乏VA会引起草鱼幼鱼眼球突出、尾鳍充血和肝功能异常，饲料中添加适量的VA会促进草鱼的生长，降低饲料系数，但过量的VA会降低草鱼幼鱼的生长速度。对草鱼体质量增长率与饲料中VA含量进行折线回归分析，可知草鱼幼鱼获得最佳生长时对VA的需要量为1 653 IU/kg，同时建议饲料中VA含量不宜超过7 950 IU/kg。 |
| 关键词: 草鱼 维生素A 饲料 生长性能 转氨酶活性 |
| DOI： |
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| 基金项目:2010年公益性行业（农业）科研专项 （201003020） |
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| Effects of vitamin A on growth performance,body composition and serum transaminase activities in the juvenile grass carp(Ctenopharyngodon idella) |
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| Abstract: |
| 【Objective】This experiment was conducted to reveal the effects of the dietary vitamin A on growth,body composition and transaminase activities in the juvenile grass carp(Ctenopharyngodon idella),to determine optimal dietary vitamin A of feed.【Method】Casein and defatted soybean were the source of protein and white dextrine was the source of carbohydrate.Retinol acetate was used as dietary vitamin A source to formulate semipurified diets at eight vitamin A levels (0,810,1 620,2 520,3 224,3 980,7 950 and 16 386 IU/kg,respectively).Each experimental diet was fed to triplicate groups of 40 fish with initial average weight (10.79±0.52) g for 12 weeks.Weight gain rate,special growth rate,feed conversion rate,body compositions and part transaminase activities in serum were detected at the end of breeding experiment.【Result】About 8.33% fish fed with diet without vitamin A exhibited exophthalmos and haemorrhages on the skin overlaying the base of the caudal fin.None of these deficiency signs was observed in fish fed with vitamin A supplemented diets.Weight gain rate and special growth rate significantly increased when dietary vitamin A level was less than 1 620 IU/kg (P＜0.05).When dietary vitamins A exceeded 7 950 IU/kg,the above indices significantly decreased(P＜0.05).The reverse trend appeared in feed conversion rate.The whole body compositions and survival rate were not affected by dietary vitamin A levels.When diet lacked vitamin A,the activity of alkaline phosphatase was significantly decreased(P＜0.05),but the activities of glutamic propylic transaminase and glutamic oxaolacetic transaminase were significantly increased in serums (P＜0.05).【Conclusion】Results of the above showed that when diet lacked vitamin A,the grass carp could be caused exophthalmos,congestion and abnormal liver function,and added vitamin A could improve growth performance,whereas,overdose vitamin A could decrease the growth.Broken-line regression analysis showed that juvenile grass carp requires a minimum of 1 653 IU/kg diet for maximal growth,and should not exceed 7 950 IU/kg diet. |
| Key words: Ctenopharyngodon idella vitamin A feed growth performance transaminase activity |
