| 摘要: |
| 【目的】分离能够降解高氯·马拉硫磷的菌株，并对其降解特性进行初步研究，以探寻高氯·马拉硫磷无公害降解方法。【方法】利用富集及驯化培养方法，从长期施用并受高氯·马拉硫磷严重污染的土壤中，分离筛选出1株能够高效降解高氯·马拉硫磷的菌株；在形态特征和生理生化鉴定的基础上，对其16S rDNA 序列进行分析，并研究了其对高氯·马拉硫磷的降解特性和最佳摇床培养条件。【结果】获得了1株能以高氯·马拉硫磷作为惟一碳源和氮源生长的菌株，经形态特征观察、生理生化特性鉴定和16S rDNA 序列分析，初步鉴定其属于假单胞菌属；系统发育树显示，该菌株与荧光假单胞菌（Pseudomonas fluorescens）的相似性最高，为99.79%，因此确定其为荧光假单胞菌。该菌株最佳摇床培养条件为：培养基初始pH值8.0，摇床转速180 r/min，培养时间24 h，培养温度30 ℃。在此培养条件下，培养2 d 的菌株对500 mg/L高氯·马拉硫磷的降解率达到67%。【结论】分离菌株对高氯·马拉硫磷有明显的降解作用，可试用于高氯·马拉硫磷污染土壤的微生物修复。 |
| 关键词: 高氯·马拉硫磷 荧光假单胞菌 降解 培养条件优化 |
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| 基金项目:陕西省科技统筹创新工程项目（2012KTCG02-02） |
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| Isolation,identification of a beta-cypermethrin malathion degrading bacterium and optimization of its cultural conditions |
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| Abstract: |
| 【Objective】The study was done to obtain beta-cypermethrin malathion degrading strains,investigate the degradation effect,and explore the non-pollution control methods.【Method】A strain was isolated from pesticide polluted soil with a long-term application of beta-cypermethrin malathion by the method of enrichment and domestication.The traditional morphology,physiological and chemical characteristics and 16S rDNA gene sequence analysis were applied to the bacteria classification.Moreover,the characteristics of degrading beta-cypermethrin malathion and the optimum shaker cultivation conditions were also studied.【Result】It can grow by using beta-cypermethrin malathion as the sole source of carbon and nitrogen,the strain was identified by morphology performance,physiological and chemical characteristics and phylogenetic analysis,the strain was identified preliminarily as the Pseudomonas;phylogenetic tree based on 16S rDNA sequences of the strain had the highest sequence identity with P.fluorescens(99.79%).Based on 16S rDNA sequence analysis as well as physiological and biochemical property,this train was identified as P.fluorescens.Further investigation indicated that the optimum shaker cultivation conditions obtained were initial pH 8.0,shaker revolution 180 r/min,culture time 24 h and culture temperature 30 ℃.Under the optimum the shaker cultivation conditions,the strain could degrade 67% beta cypermethrin malathion (500 mg/L) within 2 days.【Conclusion】It can be seen this bacterium has a striking effect on degradation of beta-cypermethrin malathion.The research provides theoretical basis for utilizing microbial remediation the beta-cypermethrin malathion contaminated soil and the best way to resolve herbicide residues. |
| Key words: beta-cypermethrin malathion Pseudomonas fluorescens degradation optimization of cultural conditions |
