| 摘要: |
| 【目的】研究复方中药（cCHM）添加剂对断奶獭兔盲肠内环境及脾脏IFN-γ、IL-10 mRNA表达的影响，探讨其对机体抗病能力的调控作用及适宜用量。【方法】选用（35±2）日龄断奶、体质量相近的獭兔144只，随机分为对照组和3个试验组，每组6个重复，每重复6只幼兔。对照组饲喂添加0.1 g/kg杆菌肽锌的日粮（不含抗生素等其他药物），试验组分别饲喂添加复方中药5 g/kg（cCHM1组）、10 g/kg（cCHM2组）、20 g/kg（cCHM3组）的日粮，60 d后，取脾脏和盲肠样品，测定盲肠微生物活力和数量，检测IFN-γ、IL-10的相对表达量。【结果】与对照组相比，cCHM2组獭兔盲肠内容物氨氮（NH3-N）浓度降低21.62%，菌体蛋白（MCP）含量提高16.81%，差异显著（P＜0.05）。复方中药抑制大肠杆菌的效果不及抗生素，但cCHM2组、cCHM3组乳酸杆菌数量较对照组分别提高18.31%和16.50%，差异显著（P＜0.05）。复方中药提高了脾脏IFN-γ、IL-10 mRNA的相对表达量，cCHM2组和cCHM3组均极显著高于对照组（P＜0.01）。【结论】复方中药添加剂可改善断奶獭兔盲肠内环境，增强机体的免疫功能，有利于断奶獭兔的肠道健康；该试验条件下复方中药添加剂的适宜用量为10 g/kg。 |
| 关键词: 复方中药 断奶獭兔 盲肠内环境 mRNA表达 |
| DOI： |
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| 基金项目:陕西省农业攻关项目（2010K01-16） |
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| Effects of dietary supplemental compound of Chinese herbal medicine on caecum inner circumstance and mRNA expression of IFN-γ and IL-10 in spleen in weaned rex rabbits |
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| Abstract: |
| 【Objective】The trial was designed to study the effects of dietary supplemental compound of Chinese herbal medicine (cCHM) on caecum inner circumstance and mRNA expression of IFN-γ and IL-10 in spleen in weaned rex rabbits.The regulatory effect of cCHM on disease resistance of weaned rex rabbits was probed,and the appropriate supplemental amount was investigated.【Method】A total of one hundred and forty-four weaned rex rabbits with (35±2) days of age and similar body weight were randomly divided into the control group and 3 experimental groups,with 6 replicates in each group and 6 rabbits per replicate.The control group was fed diet supplemented with 0.1 g/kg zinc bacitracin,and the experimental groups with 5 g/kg(cCHM1 group),10 g/kg (cCHM2 group) and 20 g/kg (cCHM3 group) cCHM,respectively.After 60 days,caecum and spleen were collected to measure the caecum microorganism vitality and quantity,and to detect mRNA relative expression of IFN-γ and IL-10 in spleen.【Result】Compared with the control group,cCHM2 group significantly decreased ammonia nitrogen (NH3-N) concentration by 21.62% and increased microbial protein (MCP) by 16.81% (P＜0.05).Although the result of inhibiting E.coli in the experimental groups was not better than the control group,both cCHM2 group and cCHM3 group increased the amount of Lactobacilli by 18.31% and 16.50% (P＜0.05),respectively.cCHM improved mRNA relative expression in spleen,compared with the control group,both cCHM2 group and cCHM3 group increased mRNA relative expression of IFN-γ and IL-10 (P＜0.01).【Conclusion】cCHM as dietary additive can improve weaned rex rabbits caecum inner circumstance and immune function,which is beneficial to intestinal health.The appropriate supplemental amount of compound Chinese herbal additive is 10 g/kg under this experimental condition. |
| Key words: compound Chinese herbal medicine weaned rex rabbits caecum inner circumstance mRNA expression |
