| 摘要: |
| 【目的】制备高效混合菌，为控制拟除虫菊酯类农药残留提供候选生物制剂。【方法】以蜡状芽孢杆菌(Bacillus cereus) ZH-3和金色链霉菌(Streptomyces aureus) HP-S-01混合菌为材料，采用单因素试验优化其生长和降解条件，高效液相色谱法(HPLC)测定其降解能力。【结果】 混合菌生长和降解拟除虫菊酯类农药的最优条件为接种量0.4 g/L、28 ℃、pH 7.5、振荡速率150 r/min，在此条件下培养72 h后，混合菌对50 mg/L氯氰菊酯的降解率达90%以上。混合菌高度耐受并降解氯氰菊酯，在氯氰菊酯初始质量浓度为100～500 mg/L时，降解率达到80%以上。混合菌最佳接种比例为1∶1，培养72 h后该比例混合菌对50 mg/L氯氰菊酯、氰戊菊酯和联苯菊酯的降解率分别为91.6%，92.5%和95.7%，比单一菌ZH 3和HP-S-01的降解率均显著提高。【结论】 混合菌对3种拟除虫菊酯类农药的降解存在协同增效作用。 |
| 关键词: 拟除虫菊酯类农药 生物降解 混合菌 协同作用 |
| DOI： |
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| 基金项目:国家自然科学基金项目(30871660) |
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| Studies on degradation characteristics of pyrethroid insecticides by one mixed strain and its synergistic effect |
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| Abstract: |
| 【Objective】The study aimed to prepare the efficient mixed strain,and to provide promising candidate biological agents for controlling pyrethroid-contaminated environments.【Method】The mixed strain were composed upon two isolated strains Bacillus cereus ZH-3 and Streptomyces aureus HP-S-01.The optimal growth and degradation conditions were determined through single-factor experiments.The degradation ability of the mixed strain was evaluated with high performance liquid chromatography (HPLC).【Result】The optimal conditions for growth and biodegradation were obtained as follows:inoculum biomass 0.4 g/L,temperature 28 ℃,pH 7.5,and with shaking speed 150 r/min.Under these conditions,the mixed strain effectively degraded cypermethrin (50 mg/L) with a degradation rate up to 90% within 72 h.Furthermore,the mixed strain tolerated and efficiently degraded high concentrations of cypermethrin (100-500 mg/L) with a degradation rate up to 80% within 72 h.The optimal inoculating rate was 1∶1 based on single strain ZH-3 and HP-S-01.At such inoculating rate,the degradation rates for cypermethrin,fenvalerate and bifenthrin were 91.6%,92.5% and 95.7%,respectively.The degradation efficiency was significantly enhanced compared with the single strain.【Conclusion】The mixed strain may have synergistic effect on degradation of cypermethrin,fenvalerate and bifenthrin. |
| Key words: pyrethroid insecticides microbial degradation mixed microorganisms synergistic effect |
