| 摘要: |
| 【目的】通过SCP3蛋白免疫荧光染色法，研究小鼠初级精母细胞减数分裂前期Ⅰ不同分裂相联会复合体的形态变化。【方法】从小鼠睾丸取曲细精管，用铺展法制片，对初级精母细胞SCP3蛋白进行免疫荧光染色，观察减数分裂前期Ⅰ不同分裂相联会复合体的形态变化。【结果】在细线期，可见短小、不连续而杂乱簇聚的SCP3蛋白片段；在偶线期，SCP3蛋白趋于明显，连续并呈线状，但没有形成完整的联会复合体；在粗线期，SCP3蛋白结构完整而清晰，小鼠初级精母细胞联会复合体共有20条，包括19条常染色体联会复合体和1条XY联会复合体；双线期，构成联会复合体的2条SCP3蛋白开始相互排斥而分离，导致联会复合体开始解体，但此时的二价体结构依然清晰可见。【结论】SCP3蛋白免疫荧光染色是研究减数分裂前期Ⅰ不同分裂相联会复合体形态变化的强有力工具。 |
| 关键词: 免疫荧光染色 SCP3蛋白 减数分裂前期Ⅰ不同亚期 小鼠初级精母细胞 |
| DOI： |
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| 基金项目:西南科技大学博士研究基金项目(06zx7121) |
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| Observation of synaptonemal complex during meiotic prophase in mouse primary spermatocytes |
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WANG Hongying,JIANG Xin
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Southwest University of Science and Technology
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| Abstract: |
| 【Objective】This study is aimed to display the morphological changes of synaptonemal complex during different substages of meiotic prophaseⅠ.【Method】Seminiferous tubules were shredded from mouse testis and the released spermatocytes were spread evenly over microscope slides.Immunofluorescence staining of SCP3 was employed to exhibit the transformation in mophology of synaptonemal complexes occurring during different substages of meiotic prophaseⅠ in mouse primary spermatocytes.【Result】Short,discontinuous and randomly converging SCP3 segments were observed in leptotene.In the subsequent zygotene,SCP3 appeared to be distinguishable,continuous and lineal,while no integral synaptonemal complexes were discerned.During pachytene,SCP3 became fully integral and clearly distinguishable.Totally 20 synaptonemal complexes were observed,including 19 autosomes and one XY bivalents.In diplotene,the SCP3 in two lateral elements began to expel and separate from each other,resulting in desynapsis while still retaining the distinguishable 20 bivalents.【Conclusion】Immunofluorescence staining of SCP3 could be one powerful technique to exhibit the morphological changes of synaptonemal complex during different substages of meiotic prophaseⅠ. |
| Key words: immunofluorescence staining SCP3 protein different sub-stages of meiotic prophaseⅠ mouse primary spermatocytes |
