| 摘要: |
| 【目的】克隆黄曲条跳甲保幼激素诱导蛋白（Juvenile hormone-inducible protein,Ps-jhip-1）基因，并分析其表达特征。【方法】克隆黄曲条跳甲Ps-jhip-1基因，对其进行系统发育分析，并对其编码蛋白进行生物信息学分析。利用荧光定量PCR方法分析Ps-jhip-1基因在黄曲条跳甲不同组织器官及1个生物钟周期内表达量的变化趋势。【结果】成功克隆了Ps-jhip-1全长cDNA，其长度为1 252 bp，开放阅读框为1 230 bp，编码409个氨基酸。Ps-jhip-1基因编码蛋白具有典型的类蛋白激酶C超家族的蛋白功能域，不具备保幼激素膜受体分子的特征。系统发育分析结果表明，Ps-jhip-1与同为鞘翅目昆虫赤拟谷盗的8种JH诱导蛋白基因聚为一支。荧光定量PCR结果表明，Ps-jhip-1基因mRNA在黄曲条跳甲雌雄成虫的多种组织器官中都有表达，其中在触角和头部的表达量相对较高，而在中足和后足的表达量相对较低，约为触角表达量的6%；Ps-jhip-1基因mRNA表达水平在1个生物钟周期内存在4个下调表达时段，但并未表现出明显的节律性特征。【结论】成功克隆了黄曲条跳甲Ps-jhip-1基因，并分析了其在不同组织及1个生物钟周期内表达量的变化。 |
| 关键词: 黄曲条跳甲 保幼激素 诱导蛋白 行为节律 荧光定量PCR |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目（31101500）；仲恺农业工程学院校级科研基金项目（G3100005） |
|
| cDNA clone and expression pattern analysis of juvenile hormone-inducible protein in Phyllotreta striolata (Fabricius) |
|
|
| Abstract: |
| 【Objective】The study was to identify juvenile hormone-inducible protein(Ps-jhip-1) of Phyllotreta striolata and to analyze its gene expression profiles. 【Method】cDNA sequence of Ps-jhip-1 was tested by clone techniques and its phylogenic relationship was analyzed.The putative protein of this cDNA was also analyzed by bioinformatic methods.Gene expression profiles in different tissues and in a circadian cycle were analyzed by real-time PCR.【Result】A full-length cDNA of juvenile hormone-inducible protein (Ps-jhip-1) was obtained.This cDNA contained 1 230 bp open reading frame (ORF) encoding 409 amino acids.It contains a PKc-like superfamily functional domain,but without any characteristics of receptor protein.Phylogenetic analysis of the complete cDNA sequence showed that the Ps-jhip-1 of P.striolata and 8 different jhip genes of Tribolium castaneum were gathered into one clade.Real-time PCR assay showed that expression profile of the Ps-jhip-1 was comparatively high in antenna and head.The expression levels of middle legs and hind legs were about 6% of that antenna.Ps-jhip-1 showed 4 down-regulated expressions during a circadian cycle,but not typical rhythm.【Conclution】cDNA sequence of Ps-jhip-1 was successfully identified,and gene expression profiles of Ps-jhip-1 in different tissues and a circadian cycle were analyzed. |
| Key words: Phyllotreta striolata(Fabricius) juvenile hormone inducible protein behavior rhythm real-time PCR |
